823427

TOYOPEARL^® AF-rProtein A HC-650F Bulk Media

phase rProtein A, bottle of 100 mL, 45 μm particle size

• 別名: TOYOPEARL^® AF-rProtein A HC-650F Bulk Media
• UNSPSCコード: 41106500

特性

• 詳細: Adsorption capacity (> 80 g/L IgG (static); >70 g/L IgG (dynamic at 5 min. residence time)); Clean in Place (CIP) (0.1 M NaOH); Phase filter (Protein A)
• 製品種目: TOYOPEARL^®
• 形状: slurry
• 包装: bottle of 100 mL
• メーカー／製品名: Tosoh 23426
• パラメーター: 3 bar max. pressure
• テクニック: LPLC: suitable
• マトリックス: hydroxylated methacrylic polymer particle platform
• マトリックス活性基: rProtein A phase
• 粒径: 45 μm
• ポアサイズ: 1000 Å
• pH: 2-13
• キャパシティ: >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time); >80 g/L adsorption capacity (IgG)(static)
• 分離法: affinity
• 保管温度: 2-8°C

詳細

詳細

TOYOPEARL AF-rProtein A HC-650F is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.
TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.
Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.

規格

Clean in place with 0.1 M NaOH.

物理的形状

72% (v/v) slurry in 20% (v/v) ethanol

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法的情報

Toyopearl is a registered trademark of Tosoh Corporation

安全性情報

• ピクトグラム: GHS02
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P210 - P233 - P240 - P241 - P242 - P243
• 危険有害性の分類: Flam. Liq. 3
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 1
• 引火点(°F): 103.1 °F
• 引火点(℃): 39.5 °C