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Merck

T2605

Sigma-Aldrich

トリプシン-EDTA 溶液

5X, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red

別名:

Trypsin-EDTA solution

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About This Item

MDL番号:
UNSPSCコード:
12352204
NACRES:
NA.78

品質水準

無菌性

sterile-filtered

製品種目

BioReagent

濃度

5X

テクニック

cell culture | mammalian: suitable

輸送温度

wet ice

保管温度

2-8°C

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アプリケーション

The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

生物化学的/生理学的作用

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

特徴および利点

StableCell trypsin is formulated as a gentle solution for cell detachment. Enzyme activity is retained when stored at 2-8°C, and our studies even show >90% activity is retained when left at room temperatures for up to 7 weeks!
  • Save Time - no more freeze or thaw cycles
  • Save Space - store at 2-8°C and free up your freezer
  • Does not need to be aliquoted
  • Only the best for your cells - manufactured in GMP environment

構成

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

調製ノート

Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution containing no Ca2+ or Mg2+.

保管および安定性

Recommended storage is 2-8°C upon arrival. During stability studies, data showed that this product retains ≥90% of its activity when stored at 37°C for up to 8 weeks.

法的情報

StableCell is a trademark of Sigma-Aldrich Co. LLC

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

T2605-VAR:
T2605-500ML:
T2605-100ML:
T2605PROC:
T2605-BULK:
T2605-PH:


試験成績書(COA)

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プロトコル

StableCell™ Trypsin solutions are designed to perform cell detachment as standard trypsin solutions do, without the need to aliquot, freeze, and thaw. This saves significant time before passaging.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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