T0813

抗腫瘍壊死因子-α ヤギ宿主抗体

affinity isolated antibody, lyophilized powder

• 別名: 抗TNF-α抗体
• UNSPSCコード: 51111800
• NACRES: NA.41

特性

• 由来生物: goat
• 品質水準: 100
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: lyophilized powder
• 化学種の反応性: human
• テクニック: immunohistochemistry: suitable; neutralization: suitable; western blot: suitable
• UniProtアクセッション番号: P01375
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TNF(7124)

詳細

詳細

Tumor Necrosis Factor-α (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of TNFR super family. The TNF/TNFR signaling axis plays a critical role in mediating either survival or apoptosis depending on the class of adaptor proteins recruited in the cell. Association of receptors with a death domain causes rapid activation of caspases and results in cell death. TNF/TNFR also results in the activation of downstream pathways involving MAPK, p38, JNK and NF-κB that play a major role in innate immunity, acute inflammatory responses and homeostasis. The TNF/TNFR axis is also important in the pathology of various autoimmune and inflammatory disorders including a variety of malignancies and Alzheimer′s disease. Anti-Tumor Necrosis Factor-α antibody is specific for human TNF-α. The antibody shows less than 10% cross reactivity with recombinant rabbit TNF-α and less than 5% cross reactivity with recombinant mouse TNF-α.

特異性

組み換えヒトTNF-αの生物活性を中和する抗体です。組み換えラットTNF-αとの交差反応性は<10%、組み換えマウスTNF-αとの交差反応性は<5%です。

免疫原

組み換えヒトTGF-α。

アプリケーション

Anti-Tumor Necrosis Factor-α (TNF- α) antibody produced in goat has been used to study the release of TNF- α from the cocultured lung carcinoma cells A427:AML-193.

Anti-Tumor Necrosis Factor-α antibody may be used for immunoblotting at a working antibody concentration of 0.1 μg/ml. For immunohistochemistry a concentration range of 5-15 μg/ml is recommended. The antibody may be used for neutralization assay; the ND50 is 0.01-0.06 μg/mL in the presence of 1.5ng/mL Recombinant Human TNF-alpha.

物理的形状

凍結乾燥品 (PBS溶液, 5%トレハロ-ス含有, 0.2μmフィルタ-濾過済み)。

調製ノート

TNF-αアフィニティ-クロマトグラフィ-で精製されています。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable