SRP5246

PAK1、活性型、GSTタグ融合 ヒト

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 別名: MGC130000, MGC130001, PAKα
• CAS番号: 177893-51-5
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected Sf9 cells
• 製品種目: PRECISIO^® Kinase
• アッセイ: ≥70% (SDS-PAGE)
• フォーム: buffered aqueous glycerol solution
• 比活性: 63-85 nmol/min·mg
• 分子量: ~95 kDa
• NCBIアクセッション番号: NM_002576
• アプリケーション: cell analysis
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PAK1(5058)

詳細

詳細

p21 (RAC1) activated kinase 1 (PAK1) is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. The gene encoding this protein is localized on human chromosome 11q13.

生物化学的／生理学的作用

p21-activated kinases (PAKs) are serine/threonine kinases that are activated by and are effectors of small GTPases, cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate (RAC). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix. The protein has a role in numerous cellular processes and has been shown to be expressed in squamous non-small cell lung carcinomas.

物理的形状

50 mM Tris-HCl(pH 7.5, 150 mM NaCl, 10 mMグルタチオン, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25%グリセロール含有)を用いた溶液として調製されています。

調製ノート

開封後はそれぞれの目的に応じて少量ずつ分注し、-70°Cで保存してください。また、取扱いや凍結融解について、繰り返しのご使用は避けてください。

法的情報

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1