SRP5223

PAD1、GSTタグ融合 ヒト

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 別名: HPAD10, PADI1, PDI, PDI1
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in baculovirus infected Sf9 cells
• アッセイ: ≥70% (SDS-PAGE)
• 形状: buffered aqueous glycerol solution
• 分子量: ~95 kDa
• NCBIアクセッション番号: NM_013358
• アプリケーション: cell analysis
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... PADI1(29943)

詳細

詳細

Peptidyl arginine deiminase 1 (PAD1) is a member of the peptidyl arginine deiminase family of enzymes. It is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway. PAD1 is expressed in the epidermis and uterus. The gene encoding this protein is localized on human chromosome 1p36.13.

生物化学的／生理学的作用

Peptidyl arginine deiminases catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The overexpression of PAD1 induced a distinctive pattern of multidrug resistance in mammalian cells and moderate resistance to ultraviolet light. PAD1 is also responsible for substrate deubiquitination during proteasomal degradation. It deiminates filaggrin in the last stages of keratinocyte differentiation. Thus, PAD1 has a role in epidermis function and homeostasis. The protein has been genetically associated with rheumatoid arthritis.

物理的形状

50 mM Tris-HCl(pH 7.5, 150 mM NaCl, 10 mMグルタチオン, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25%グリセロール含有)を用いた溶液として調製されています。

調製ノート

開封後はそれぞれの目的に応じて少量ずつ分注し、-70°Cで保存してください。また、取扱いや凍結融解について、繰り返しのご使用は避けてください。

アナリシスノート

This protein is not assayed for enzymatic activity.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable