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由来生物
human
品質水準
リコンビナント
expressed in E. coli
アッセイ
≥90% (SDS-PAGE)
形状
aqueous solution
比活性
≥200 units/mg protein
分子量
74 kDa
UniProtアクセッション番号
アプリケーション
genomic analysis
輸送温度
dry ice
保管温度
−20°C
遺伝子情報
human ... PADI4(23569)
詳細
Peptidyl Arginine Deiminases (PADs) perform post-translational deiminations of proteins. PADs are calcium-dependent enzymes that catalyze the conversion of L-arginine residues to L-citrulline. The catalyzed reaction is as follows:
2+
Ca Protein--[L-arginine] + H2O ––––> Protein--[L-citrulline]
There are five mammalian PADs sub-types, which differ in substrate specificity and tissue distribution. PAD enzymes are highly homologous, with 50–60% sequence similarity.1 PADs play important roles in gene regulation by citrullination of arginine residues on histones H3, H2A, and H4. Overexpression of PADs has been found in conditions such as rheumatoid arthritis, Alzheimer′s disease, multiple sclerosis, lupus,Parkinson′s disease, and cancer.2,3
PAD4 is widely expressed in the immune system with expression pathology in various tumor and cancer cell lines.5 In cells, PAD4 can be found in the cytoplasm and nucleus. PAD4 activity has been correlated to the development and progress of Rheumatoid Arthritis (RA).2,5 A study in mice has indicated a mechanism for the development of RA by anticitrullin autoimmunity.6
This recombinant human PAD4 is expressed in Escherichia coli as a fusion protein. The fusion partner is removed by HRV3C protease digestion, followed by chromatographic purification.
2+
There are five mammalian PADs sub-types, which differ in substrate specificity and tissue distribution. PAD enzymes are highly homologous, with 50–60% sequence similarity.1 PADs play important roles in gene regulation by citrullination of arginine residues on histones H3, H2A, and H4. Overexpression of PADs has been found in conditions such as rheumatoid arthritis, Alzheimer′s disease, multiple sclerosis, lupus,Parkinson′s disease, and cancer.2,3
PAD4 is widely expressed in the immune system with expression pathology in various tumor and cancer cell lines.5 In cells, PAD4 can be found in the cytoplasm and nucleus. PAD4 activity has been correlated to the development and progress of Rheumatoid Arthritis (RA).2,5 A study in mice has indicated a mechanism for the development of RA by anticitrullin autoimmunity.6
This recombinant human PAD4 is expressed in Escherichia coli as a fusion protein. The fusion partner is removed by HRV3C protease digestion, followed by chromatographic purification.
生物化学的／生理学的作用
Calcium is required for peptidylarginine deiminase activity in vitro.
単位の定義
One unit will produce 1 μmole of N-a-benzoylcitrulline ethyl ester from BAEE per hour at 37 °C at pH 7.7.
物理的形状
Supplied as an aqueous solution in 10 mM Tris (pH 7.5), 500 mM NaCl, 1 mM EDTA, and 1 mM DTT.
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
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Jan Code
SAE0086-BULK:
SAE0086-25UN:
SAE0086-VAR:
SAE0086-25UN-PW:
試験成績書（COA）
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