SRP5148

Tubulin、GSTタグ融合 ヒト

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 別名: M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB, TUBB5
• CAS番号: 1622402-26-9
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥70% (SDS-PAGE)
• フォーム: buffered aqueous glycerol solution
• 分子量: ~76 kDa
• NCBIアクセッション番号: NM_178014
• アプリケーション: cell analysis
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... TUBB(203068)

詳細

詳細

Tubulin-β 1 (TUBB1) protein is a major constituent of microtubules. Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13- or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both α and β tubulin monomers. It is found in hematopoietic cells and platelet marginal bands. The gene encoding TUBB1 is localized on human chromosome 6p21.

アプリケーション

GST tagged tubulin has been used in Western blotting and in mass spec as a control.

生物化学的／生理学的作用

Tubulin-β 1 (TUBB1) interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function. It has a role in the formation of the cytoskeleton. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance.

物理的形状

50 mM Tris-HCl(pH 7.5, 150 mM NaCl, 10 mMグルタチオン, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25%グリセロール含有)を用いた溶液として調製されています。

調製ノート

開封後はそれぞれの目的に応じて少量ずつ分注し、-70°Cで保存してください。また、取扱いや凍結融解について、繰り返しのご使用は避けてください。

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable