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由来生物
human
リコンビナント
expressed in E. coli
アッセイ
≥55% (SDS-PAGE)
形状
aqueous solution
分子量
52 kDa
包装
pkg of 50 μg
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−70°C
遺伝子情報
human ... ATAT1(79969)
詳細
Human α tubulin acetyltransferase 1, or ATAT1, (GenBank Accession No. NM_001031722 (var1)), amino acids 2 – 224 with N-terminal GST-tag, MW=52 kDa, expressed in an E. coli expression system.
The αTAT1 (α tubulin acetyltransferase 1) protein sequence of αTAT1 is highly conserved among eukaryotes. αTAT1 is a member of Gcn5 (general control non-depressible 5)-related N-acetyltransferase superfamily and is mostly present in ciliated organisms. It has also been identified in Tetrahymena, nematodes, zebrafish and mice.
The αTAT1 (α tubulin acetyltransferase 1) protein sequence of αTAT1 is highly conserved among eukaryotes. αTAT1 is a member of Gcn5 (general control non-depressible 5)-related N-acetyltransferase superfamily and is mostly present in ciliated organisms. It has also been identified in Tetrahymena, nematodes, zebrafish and mice.
生物化学的／生理学的作用
ATAT1 (α tubulin acetyltransferase 1) catalyses the acetylation of lysine 40 of α-tubulin and microtubule. ATAT1 expression is essential for microtubule stabilization and mediates cilium formation. Upart from acetylation, αTAT1 interacts with doublecortin (a microtubule associated protein) to degrade microtubules that are not aligned with acetyltransferase activity. αTAT1 regulates the Wnt1 signalling pathway via β-catenin and promotes tumorigenesis. Overexpression of the ATAT1 gene is observed in colon cancer and is known to contribute to its progression.
保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
SRP0309-50UG:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Tubulin acetylation: responsible enzymes, biological functions and human diseases.
Li L and Yang X J
Cellular and Molecular Life Sciences, 72(22), 4237-4255 (2015)
R A Lloyd et al.
Analytical biochemistry, 216(1), 42-46 (1994-01-01)
An assay for alpha-tubulin acetyltransferase (TAT) activity based on affinity isolation of labeled acetylated alpha-tubulin was developed for use with crude subcellular fractions. Microtubules were polymerized and immobilized on an anti-alpha-tubulin-agarose and then incubated with the subcellular fraction and [3H]acetyl-coenzyme
Genetic disruption of tubulin acetyltransferase, ?TAT1, inhibits proliferation and invasion of colon cancer cells through decreases in Wnt1/?-catenin signaling.
Oh S, et al.
Biochemical and Biophysical Research Communications, 482(1), Aug-A14 (2017)
Microtubules acquire resistance from mechanical breakage through intralumenal acetylation.
Xu Z, et al.
Science, 356(6335), 328-332 (2017)
A Mec17-Myosin II effector axis coordinates microtubule acetylation and actin dynamics to control primary cilium biogenesis.
Rao Y, et al.
PLoS ONE, 9(12), e114087-e114087 (2014)
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