SRP4896

TSLP ヒト

recombinant, expressed in E. coli, ≥97% (SDS-PAGE), ≥97% (HPLC)

• 別名: 胸腺間質性リンパ球新生因子
• UNSPSCコード: 51111800
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥97% (HPLC); ≥97% (SDS-PAGE)
• フォーム: lyophilized
• 有効性: <0.3 ng/mL ED₅₀
• 分子量: ~15.0 kDa
• 包装: pkg of 20 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 不純物: endotoxin, tested
• NCBIアクセッション番号: Q969D9
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... TSLP(85480)

詳細

詳細

Thymic stromal lymphopoietin (TSLP) is a hemopoietic cytokine. It is part of the interleukin-2 (IL-2) cytokine family. It is mainly expressed in the heart, liver and prostate. TSLP is related in its biological activities to interleukin-7 (IL-7) and binds with the heterodimeric receptor complex consisting of interleukin-7 receptor α chain & the TSLP receptor. Recombinant human TSLP produced in Escherichia coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 15kDa.

アプリケーション

TSLP human has been used in immunohistochemistry.

生物化学的／生理学的作用

Thymic stromal lymphopoietin (TSLP) signals throughout a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the interleukin-7 (IL-7) receptor a chain. TSLP impacts myeloid cells and thus induces the discharge of T cell-attracting chemokines from monocytes and increases the growth of CD11c⁺ dendritic cells. Similar to IL-7, TSLP enhances phosphorylation of signal transducer and activator of transcription 3 and 5 (STAT3 and STAT5), though uses kinases excluding Janus kinases (JAKs) for its activation. TSLP induces the release of T cell-attracting chemokines such as thymus and activation-regulated chemokine (TARC) and macrophage-derived chemokine (MDC) from monocytes and triggers CD11c⁺ dendritic cells. TSLP activated dendritic cells primes naive T cells to manufacture pro-allergic cytokines such as interleukin-4, interleukin-5, interleukin-13 and tumor necrosis factor-α (TNF-α), whereas down-regulating interleukin-10 and interferon-γ (IFN-γ) play a role in the initiation of allergic inflammation.

物理的形状

20 mMリン酸ナトリウム(pH 7.4, 130 mM NaCl)を含む濃縮液(1 mg/mL)の凍結乾燥品。

再構成

バイアルは、開封前に遠心してください。凍結融解の繰り返しは避けてください。

Reconstitute in sterile dH₂O to a concentration of 0.1 -1 mg/mL and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable