F3668

Monoclonal Anti-CD16−FITC antibody produced in mouse

clone 3G8, purified immunoglobulin, buffered aqueous solution

• 別名: Anti-CD16-II-Fluorescein isothiocyanate, Anti-CD16A-Fluorescein isothiocyanate, Anti-FCG3-Fluorescein isothiocyanate, Anti-FCGR3-Fluorescein isothiocyanate, Anti-FCGRIII-Fluorescein isothiocyanate, Anti-FCR-10-Fluorescein isothiocyanate, Anti-FCRIII-Fluorescein isothiocyanate, Anti-FCRIIIA-Fluorescein isothiocyanate, Anti-FcGRIIIA-Fluorescein isothiocyanate, Anti-IGFR3-Fluorescein isothiocyanate, Anti-IMD20-Fluorescein isothiocyanate
• MDL番号: MFCD00675220
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: mouse
• 結合体: FITC conjugate
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: 3G8, monoclonal
• 形状: buffered aqueous solution
• 化学種の反応性: human
• 保管条件: protect from light
• テクニック: flow cytometry: 10 μL using 1 × 10⁶ cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: P08637
• 輸送温度: wet ice
• 保管温度: 2-8°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FCGR3A(2214) , FCGR3B(2215)

詳細

詳細

Human CD16 or FCγRIII is a 50-70kDa membrane glycoprotein expressed by neutrophils, eosinophils, cultured monocytes, natural killer (NK) cells, tissue macrophages subpopulation, and on a small subset of T cells that binds aggregated but not monomeric human IgG. It is also known as FcR3, the low affinity receptor for complexed IgG. It comprises of two truncated Ig-like cytoplasmic domains.

Monoclonal Anti-CD16 (mouse IgG1 isotype) is derived from the 3G8 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized CD2F1 mouse.

特異性

ナチュラルキラ-(NK)細胞, 顆粒球, マクロファ-ジ亜集団に発現するヒトCD16抗原を認識します。本抗体はNA1, NA2好中球とNKリンパ球の両方に反応します。 凍結組織切片の好中球を強く標識します。肝臓クッパ-細胞もわずかに染色されます。本抗体により認識されるエピト-プはFCγ-RIIIのIgG結合部位の近傍に存在するとおもわれます。本抗体は顆粒球への可溶性免疫複合体の結合を機能的にブロックし、E-IgGロゼットを阻害します。好中球におけるCa²⁺の増加を引き起こします。

免疫原

生存ヒト多形核細胞

アプリケーション

Monoclonal Anti-CD16-FITC antibody is suitable for receptor blocking in flow cytometry analysis.

Monoclonal Anti-CD16-FITC antibody produced in mouse has been used in direct immunofluorescent staining.

生物化学的／生理学的作用

Cluster of differentiation 16 (CD16) induces Ca²⁺ increase in neutrophils. It strongly labels neutrophils in tissue frozen sections. Liver kupffer cells are weakly stained. The antibody functionally blocks binding of soluble immune complexes to granulocytes and inhibits E-IgG rosettes.

Human CD16 plays a pivotal role in immune responses by linking the humoral immune system with cellular effector functions. CD16 plays an important role in natural killer (NK)-cell function and in peripheral blood mononuclear cells (PBMCs). In the FcyRIII-expressing lymphocytes the NK cell activity is highly influenced through FcyRIII. CD16 exists as a transmembrane form in NK cells, macrophages and cultured monocytes with a 25 amino acid cytoplasmic tail which further connects with other receptors. The transmembrane form binds complexed IgG and mediates phagocytosis and antibody-dependent cellular cytotoxicity (ADCC).

ターゲットの説明

CD16抗原(別名: IgG複合体低親和性レセプタ-, Fcγ-RIII )は、ナチュラルキラ-(NK)細胞、マクロファ-ジ亜集団をはじめ、顆粒球にも弱い発現がみられます。ヒトCD16分子では二つの切断型Ig様ドメインが認められます。NK細胞とマクロファ-ジでは25アミノ酸の細胞質テ-ルを有する膜貫通型(50~80 kDa)が存在します。このフォ-ムは他のレセプタ-(FcεRIγまたはTcRξ鎖)と非共有結合的に会合します。顆粒球のヒトCD16分子は、グリコシルホスファチジルイノシト-ル(GPI)との結合型になっています。

物理的形状

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)。

調製ノート

フルオレセインイソチオシアネ-トアイソマ-I(FITC)とのコンジュゲ-ションにより作製しています。この緑色色素は励起波長495 nm, 蛍光波長525 nmでの測定が効果的です。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)