SRP4324

Apo-SAA ヒト

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• 別名: SAA, SAA1, SAA2, アミロイドタンパク質A, アミロイド線維タンパク質AA, 血清アミロイドAタンパク質
• MDL番号: MFCD01865610
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 形状: lyophilized
• 分子量: ~11.5 kDa
• 包装: pkg of 50 μg
• 保管条件: avoid repeated freeze/thaw cycles
• テクニック: protein expression: suitable
• 不純物: endotoxin, tested
• NCBIアクセッション番号: P02735
• 輸送温度: wet ice
• 保管温度: −20°C
• 遺伝子情報: human ... SAA1(6288)

詳細

詳細

ヒトapo-SAAは、主として高密度リポタンパク質(HDL)と結合して循環する104アミノ酸のポリペプチドです。apo-SAAの通常の血漿レベルは1～5 μg/mLですが、炎症性の刺激を受けると24時間以内で500～1,000倍に増加することから、このような条件下ではHDLの中で最も豊富なアポリポプロテイン分子となります。ヒトSAA遺伝子は、18アミノ酸のN末端シグナル配列を含む122アミノ酸をコードしています。

Research Area: IMMUNO AND CKS
Serum amyloid A (SAA) proteins are a group of apolipoproteins produced in response to cytokines released by activated monocytes/macrophages. SAA is a highly conserved acute-phase protein primarily synthesized by the liver.

アプリケーション

Apo-SAA human has been used to culture and stimulate RAW264.7 macrophages to study its effects on visfatin expression.

Apo-serum amyloid A has been used to study its effect on the expression of Visfatin in macrophages.

生物化学的／生理学的作用

Serum amyloid A (SAA) is an equally sensitive marker for the acute phase as C-reactive protein (CRP). It is the most sensitive non-invasive biochemical indicator for allograft rejection. The function of SAA as a cytokine-like protein has been acknowledged in cell-cell communication and as a feedback mechanism in inflammatory, immunologic, neoplastic, and protective pathways. SAA plays a crucial role in the regulation and potentially the spread of the initial acute phase response.

Serum amyloid A (SAA) is found normally at concentrations of 0.1μM in blood. However, its concentration increases up to 1000-fold under stress. It has been associated with several chronic inflammatory diseases, such as atherosclerosis. It is found to participate in the inflammatory response by facilitating chemotaxis, migration, and adhesion of inflammatory cells, particularly monocytes/macrophages. SAA is also found to induce the synthesis and secretion of multiple kinds of inflammatory cytokines, such as TNF-α (tumor necrosis factor-α), IL-1β (interleukin-1β), MCP-1 (monocyte chemoattractant protein-1), and Lp-PLA2 (lipoprotein-associated phospholipase A2).

物理的形状

凍結乾燥品(ろ過滅菌済み、添加剤不含)。

再構成

バイアルは、開封前に遠心してください。凍結融解の繰り返しは避けてください。

Reconstitute in 0.1% acetic acid to a concentration of 1 μg/ul. This solution can then be diluted into other aqueous buffers.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable