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由来生物
human
リコンビナント
expressed in E. coli
アッセイ
≥98% (HPLC)
≥98% (SDS-PAGE)
形状
lyophilized
分子量
20.0 kDa
包装
pkg of 50 μg
テクニック
cell culture | mammalian: suitable
不純物
<0.1 EU/μg endotoxin, tested
色
white to off-white
UniProtアクセッション番号
輸送温度
wet ice
保管温度
−20°C
遺伝子情報
human ... TNFRSF11B(4982)
関連するカテゴリー
詳細
Osteoprotegerin (OPG) is a member of the TNFR (tumor necrosis factor receptor) superfamily that can act as a decoy receptor for RANKL (receptor activator of nuclear factor κ-B ligand). The gene is mapped to human chromosome 8q24. The primary structure of OPG protein consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney, liver, spleen, prostate, lymph node and bone marrow. It is secreted both as a monomeric and a dimeric protein. Recombinant human OPG is a soluble 20kDa protein containing 174 amino acid residues.
アプリケーション
Osteoprotegerin (OPG) human has been used as a supplement in human cell culture during cytokine production.
生物化学的／生理学的作用
Binding of soluble osteoprotegerin (OPG) to sRANKL (soluble receptor activator of nuclear factor κ-B ligand) inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Changes in the level of serum OPG are associated with atherosclerosis and cardiovascular disorders. It might also be associated with malignancy and plays a regulatory role in multiple myeloma, prostate, breast, bladder and gastric carcinoma.
シーケンス
METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK
物理的形状
凍結乾燥品(10 mM Tris、pH, 7.5、75 mM NaCl)
再構成
バイアルは、開封前に遠心してください。5 mM Tris(pH 7.5)で濃度0.1～1.0 mg/mLに復元してください。ボルテックスは行なわないでください。水溶液は2～8°Cで最長1週間保存できます。長期保存には、担体タンパク質(0.1% BSAなど)含有バッファーでさらに希釈し、1回の使用量ずつに分注して-20～-80°Cで保存することを推奨します。
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Osteoprotegerin secreted by inflammatory and invasive breast cancer cells induces aneuploidy, cell proliferation and angiogenesis.
Goswami S and Sharma-Walia N
BMC Cancer, 15, 935-935 (2015)
Polymorphisms in three genes are associated with hemorrhagic stroke.
Brain and Behavior, 5, e00395-e00395 (2015)
The tumor necrosis factor superfamily member RANKL suppresses effector cytokine production in group 3 innate lymphoid cells
Bando JK, et al.
Immunity, 48(6), 1208-1219 (2018)
Functions of RANKL/RANK/OPG in bone modeling and remodeling.
Boyce BF and Xing L
Archives of Biochemistry and Biophysics, 473, 139-146 (2008)
H Hsu et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(7), 3540-3545 (1999-03-31)
A receptor that mediates osteoprotegerin ligand (OPGL)-induced osteoclast differentiation and activation has been identified via genomic analysis of a primary osteoclast precursor cell cDNA library and is identical to the tumor necrosis factor receptor (TNFR) family member RANK. The RANK
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