O8137

オステオポンチン/Fcキメラ マウス由来

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• 別名: OCIF, OPG
• MDL番号: MFCD01867131
• UNSPSCコード: 12352202
• PubChem Substance ID: 24898055
• NACRES: NA.32

特性

• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in NSO cells
• アッセイ: >95% (SDS-PAGE)
• 形状: lyophilized powder
• 有効性: 25-50 ng/mL ED₅₀
• 分子量: 100 kDa by SDS-PAGE (reducing); calculated mol wt 70.9 kDa
• 包装: pkg of 100 μg
• 不純物: endotoxin, tested
• UniProtアクセッション番号: O08712
• 保管温度: −20°C
• SMILES記法: CCCCCCCCCCC#CCCCCCC(O)=O
• InChI: 1S/C18H32O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h2-10,13-17H2,1H3,(H,19,20)
• InChI Key: XXUPLYBCNPLTIW-UHFFFAOYSA-N
• 遺伝子情報: mouse ... Tnfrsf11b(18383)

詳細

詳細

Osteoprotegerin (OPG) is a member of the TNFR (tumor necrosis factor receptor) superfamily that can act as a decoy receptor for RANKL (receptor activator of nuclear factor κ-B ligand) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). The primary structure of OPG protein consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney and liver. It is secreted both as a monomeric and a dimeric protein.

生物化学的／生理学的作用

OPGは、腫瘍壊死因子受容体ス-パ-ファミリ-に属する可溶性の分泌物であり、細胞の結合に関与する明確なモチ-フを欠いています。破骨細胞の形成を調節するとともに、骨吸収や骨リモデリングの調節にも関与しています。ラットOPGに対するヒトOPGは約84%、マウスOPGは約94%、同一性のアミノ酸配列を共有しています。

Binding of soluble osteoprotegerin (OPG) to sRANKL (soluble receptor activator of nuclear factor κ-B ligand) inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Changes in the level of serum OPG are associated with atherosclerosis and cardiovascular disorders. It might also be associated with malignancy and plays a regulatory role in multiple myeloma, prostate, breast, bladder and gastric carcinoma. OPG plays a vital role in bone metabolism and is involved in inhibition of ovariectomy-associated bone loss in rats.

物理的形状

凍結乾燥品(0.2μmフィルタ-で減菌濾過済み, PBS溶液)。

アナリシスノート

生物活性は、50ng/mlの組み換えヒトTRAULで処理されたマウスL-929細胞のアポト-シスに対する中和能で測定しています。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable