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由来生物
human
リコンビナント
expressed in E. coli
アッセイ
≥90% (SDS-PAGE)
形状
aqueous solution
分子量
53 kDa
包装
pkg of 100 μg
保管条件
avoid repeated freeze/thaw cycles
濃度
2.9 mg/mL
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−70°C
遺伝子情報
human ... PLK1(5347)
詳細
Human Plk1 polo box domain (GenBank Accession No. NM_005030), amino acids 367-end with N-terminal GST-tag, MW=53 kDa, expressed in an E. coli expression system.
Polo-like kinase 1 (PLK1) belongs to the PLK family. It comprises polo-box domain (PBD). PBD, in turn, contains polo boxes (PB1 and PB2) with a 6-stranded β sandwich and an α helix as structural elements. The PLK1 gene is mapped to human chromosome 16p12.2.
アプリケーション
PLK1 Polo Box Domain active human has been used in the in vitro binding assay with various synthetic peptides coupled to streptavidin beads. It has also been used in binding studies with fluorescein-labeled substrate peptide 5-carboxyfluorescein-GPMQSpTPLNG to test the effect of various inhibitors.
生物化学的／生理学的作用
Polo-like kinase 1 (PLK1) is a mitotic kinase that mediates the regulation of various events in mitosis including chromosome segregation, cytokinesis, centrosome maturation, and separation. It activates cell division cycle 25 (CDC25) phosphatase which further regulates mitotic entry. Elevated expression of PLK1 is observed in many tumors.
保管分類コード
10 - Combustible liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Sugunadevi Sakkiah et al.
PloS one, 9(7), e101405-e101405 (2014-07-19)
The polo-like kinase 1 (Plk1) is a critical regulator of cell division that is overexpressed in many types of tumors. Thus, a strategy in the treatment of cancer has been to target the kinase activity (ATPase domain) or substrate-binding domain
Sara Abdelfatah et al.
Journal of advanced research, 16, 145-156 (2019-03-23)
Polo-like kinase 1 (PLK1), a member of the Polo-like kinase family, plays an important regulatory role in mitosis and cell cycle progression. PLK1 overexpression is correlated with tumourigenesis and poor prognosis in cancer patients. Therefore, the identification of novel compounds
Zsofia A Novak et al.
Developmental cell, 37(6), 545-557 (2016-06-22)
Centrosomes and cilia are organized by a centriole pair comprising an older mother and a younger daughter. Centriole numbers are tightly regulated, and daughter centrioles (which assemble in S phase) cannot themselves duplicate or organize centrosomes until they have passed
Begoña García-Alvarez et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 62(Pt 4), 372-375 (2006-04-04)
Polo-like kinase (Plk1) is crucial for cell-cycle progression via mitosis. Members of the Polo-like kinase family are characterized by the presence of a C-terminal domain termed the Polo-box domain (PBD) in addition to the N-terminal kinase domain. The PBD of
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