SRP0230
XIAP Active human
recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)
別名:
API3, BIRC4, IAP3, Baculoviral IAP repeat-containing protein 4 (BIRC4), E3 ubiquitin-protein ligase XIAP (MIHA), IAP-like protein (ILP1), Inhibitor of apoptosis protein 3, X-linked inhibitor of apoptosis
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About This Item
UNSPSCコード:
12352200
NACRES:
NA.32
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詳細
XIAP (X-linked inhibitor of apoptosis) is an anti-apoptotic protein belonging to the inhibitor of apoptosis protein (IAP) family, an evolutionary conserved protein family. XIAP contains three tandem copies of BIR (baculovirusIAP repeat) domain, which is composed of ∼70 residues. IAP family members also contain a RING (really interesting new gene) zinc finger domain in their C-terminals.
アプリケーション
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生物化学的／生理学的作用
XIAP (X-linked inhibitor of apoptosis) is the most potent caspase-interacting protein and inhibits both intrinsic and extrinsic pathways of apoptosis. Its up-regulation in tumor cells is linked with resistance to chemotherapy. In inflammatory breast cancer (IBC) this protein is linked with resistance to antibody-dependent cellular cytotoxicity (ADCC) driven by cetuximab and trastuzumab. Embelin, an inhibitor of XIAP, functions as an anti-tumor agent in myeloma cell line (HL-60) by suppressing the expression of XIAP. Elevated expression of XIAP in rectal cancer is associated with chemotherapy resistance.
単位の定義
1ユニットは、37°Cで1分あたり1 pmolのユビキチンを基質へ転移するために必要な酵素量と定義されています。
物理的形状
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol, and 3 mM DTT.
調製ノート
氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
X-linked inhibitor of apoptosis protein mediates tumor cell resistance to antibody-dependent cellular cytotoxicity.
Evans MK et al
Cell Death & Disease, 7, e2073-e2073 (2016)
A single BIR domain of XIAP sufficient for inhibiting caspases.
Takahashi R et al
The Journal of Biological Chemistry, 273(14), 7787-7790 (1998)
Synergism between NF-kappa B inhibitor, celastrol, and XIAP inhibitor, embelin, in an acute myeloid leukemia cell line, HL-60.
Pazhang Y et al
Journal of Cancer Research and Therapeutics, 12(1), 155-160 (2016)
Jun Zhou et al.
Cancer immunology, immunotherapy : CII, 68(8), 1331-1340 (2019-07-19)
Expression of inhibitors of apoptosis protein (IAP) family members is associated with poor prognosis in cancer patients. Immunity to ML-IAP (livin) and survivin has been well studied in patients with a variety of tumors. XIAP, the most potent inhibitor of
Arun Murali et al.
Cell death & disease, 8(6), e2900-e2900 (2017-07-01)
Rho GTPases control fundamental cellular processes and Cdc42 is a well-studied member of the family that controls filopodia formation and cell migration. Although the regulation of Cdc42 activity by nucleotide binding is well documented, the mechanisms driving its proteostasis are
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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