SRP0120

Sirtuin 6 human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 別名: SIR2L6, SIRT6
• UNSPSCコード: 12352200
• NACRES: NA.32

特性

• 由来生物: human
• リコンビナント: expressed in E. coli
• アッセイ: ≥70% (SDS-PAGE)
• 形状: aqueous solution
• 分子量: 65 kDa
• 包装: pkg of 100 μg
• 保管条件: avoid repeated freeze/thaw cycles
• 濃度: >0.02 mg/mL
• NCBIアクセッション番号: NM_016539
• UniProtアクセッション番号: Q8N6T7
• 輸送温度: dry ice
• 保管温度: −70°C
• 遺伝子情報: human ... SIRT6(51548)

詳細

詳細

Sirtuin 6 (Sir6) is encoded by the gene mapped to human chromosome 19p13.3, a region linked with development of acute leukemia. Sir 6 is mainly expressed in bone cells and in the ovaries, but it is absent in the bone marrow. Sirt6 is a chromatin-associated protein localized specifically in nucleus.
Human Sirtuin 6, GenBank Accession No. NM_016539), full length with N-terminal ST tag, MW = 65kDa, expressed in Escherichia coli expression system.

アプリケーション

酵素キネティクスの研究をはじめ、インヒビターのスクリーニングや選択性プロファイリングにもご利用いただけます。

生物化学的／生理学的作用

Sirtuin 6 (Sir6) is a mono-ADP-ribosyltransferase, which catalyzes the transfer of ADP-ribose moiety from NAD to itself and histones. It has been implicated in the regulation of DNA repair, genomic stability and telomere integrity, gene expression, and metabolism. In addition, it also prevents age-related disorders and premature aging. Sir6 plays a vital role in the inhibition of nuclear factor-κB (NF-κB)–mediated inflammatory responses in rheumatoid joints. Hence, Sir6 is considered to be a potent target for treatment of rheumatoid arthritis (RA). Sir6 is also majorly implicated in the regulation of glucose and fat metabolism. Sir6 prevent cardiac hypertrophy in vitro via inhibiting NF-κB-dependent transcriptional activity and this effect is relied on its deacetylase activity.

物理的形状

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 20% glycerol.

調製ノート

氷上で解凍してください。最初に解凍した際、チューブを軽く遠心して内容物を完全に回収してください。酵素液は一回の使用量ずつ分注してください。分注された希釈前の酵素を-70°Cで保存してください。注記:酵素は凍結解凍の繰り返しに非常に弱いので、注意してください。