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由来生物
mouse
品質水準
抗体製品の状態
purified immunoglobulin
抗体製品タイプ
primary antibodies
クローン
GST-2, monoclonal
フォーム
buffered aqueous solution
濃度
~1 mg/mL
テクニック
ELISA: suitable
dot blot: suitable
immunoblotting: 0.5 μg/mL
immunofluorescence: suitable
immunoprecipitation (IP): suitable
アイソタイプ
IgG2b
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
詳細
Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the hybridoma GST-2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant GST fusion protein. Glutathione-S-Transferase (GST) is a 26 kDa protein found in eukaryotic cells. It belongs to the phase II detoxification enzymes family.
免疫原
purified recombinant GST fusion protein
アプリケーション
Anti-Glutathione-S-Transferase (GST) antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunoprecipitation
- dot blotting
- autoradiography
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
生物化学的/生理学的作用
Glutathione-S-Transferase (GST) helps to catalyze the conjugation of electrophilic substrates to reduced form of glutathione (GSH). Genetic engineering uses glutathione-S-transferase (from parasitic helminth Schistosoma japonicum) to create the GST gene fusion system for production of GST-labeled recombinant proteins. GST-fusion proteins were successfully demonstrated in structure- function studies involving protein-protein and DNA protein interactions via GST pull down assays.
物理的形状
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
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Methods in Molecular Biology, 261, 175-86 (2004)
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Immunology Letters, 79, 197-202 (2001)
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