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Merck

SAB4200503

Sigma-Aldrich

Anti-PSPC1 antibody, Mouse monoclonal

enhanced validation

clone 1L4, purified from hybridoma cell culture

別名:

Monoclonal Anti-PSP1, Monoclonal Anti-PSPC1 antibody produced in mouse, Monoclonal Anti-paraspeckle component 1

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

結合体

unconjugated

抗体製品の状態

purified from hybridoma cell culture

抗体製品タイプ

primary antibodies

クローン

1L4, monoclonal

形状

buffered aqueous solution

分子量

antigen ~60 kDa

化学種の反応性

human, rat, mouse

強化検証

independent
Learn more about Antibody Enhanced Validation

濃度

~1.0 mg/mL

テクニック

indirect immunofluorescence: 5-10 μg/mL using Human HeLa cells.
western blot: 0.5-1 μg/mL using Whole extracts of mouse Hepa1-6 cells.

アイソタイプ

IgG1

UniProtアクセッション番号

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... PSPC1(55269)
mouse ... Pspc1(66645)
rat ... Pspc1(305910)

詳細

Monoclonal Anti-PSPC1 (mouse IgG1 isotype) is derived from the hybridoma 1L4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1. DBHS (Drosophila behavior, human splicing) protein family, consisting also of NONO (nonPOU-domain-containing, octamer binding protein) and SFPQ (splicing factor, praline- and glutamine-rich). NONO, paraspeckle component 1 (PSPC1) and SFPQ are the core protein components of paraspeckles. These multi-functional nuclear proteins, under normal conditions, cycle between the nucleoplasm, paraspeckles and the nucleolus.
Paraspeckle component 1 (PSPC1) is encoded by the gene mapped to human chromosome 13q12.11. The encoded protein is a member of DBHS (Drosophila Behavior Human Splicing) family of proteins.

免疫原

Synthetic peptide corresponding to a sequence at the C-terminal region of mouse PSPC1, conjugated to KLH. 1 The corresponding sequence is identical in rat and human PSPC1.

アプリケーション

Monoclonal Anti-PSPC1 antibody produced in mouse has been used in immunohistochemistry, immunoblotting and immunofluorescence.

生物化学的/生理学的作用

Drosophila behavior, human splicing (DBHS) proteins bind both double- and single-stranded DNA and RNA, and are involved in various aspects of RNA and DNA metabolism, such as transcription, pre-mRNA 3′ processing, transcription termination and mRNA splicing. DBHS proteins regulate circadian rhythm and carcinogenesis. PSPC1 activates androgen receptor mediated transcription. PSPC1 forms a heterodimer with NONO that localizes to paraspeckles in a RNA polymerase II-dependent manner.
Since paraspeckle component 1 (PSPC1) has lower background nucleoplasmic signal in various cell types compared to other members of DBHS (Drosophila Behavior Human Splicing) family of proteins, it is frequently used as a marker for paraspeckles. The encoded protein plays a vital role in regulation of transcription. Deletion of the gene is associated with the hindered psychomotor development, muscle hypotonia, unilateral microphthalmia with ptosis, congenital eye malformation and facial dysmorphic features.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

nwg

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SAB4200503-VAR:
SAB4200503-BULK:
SAB4200503-200UL:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation
Passon DM, et al.
Proceedings of the National Academy of Sciences of the USA, 109(13), 4846-4850 (2012)
First-trimester euploid miscarriages analysed by array-CGH.
Viaggi CD
Journal of applied genetics, 54, 353-359 (2013)
Neat1 in hematopoietic stem cells
Fallik N, et al.
Oncotarget, 8(65), 109575-109575 (2017)
Noam Fallik et al.
Oncotarget, 8(65), 109575-109586 (2018-01-10)
Hematopoietic Stem Cells (HSCs) generate blood and immune cells through a hierarchical process of differentiation. Genes that regulate this process are of great interest for understanding normal and also malignant hematopoiesis. Surprisingly, however, very little is known about long-non-coding RNAs
Paraspeckles
Fox A H
Cold Spring Harbor Perspectives in Biology, 2 (2010)

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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