SAB4200283

Anti-Tensin 1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• 別名: Anti-TNS1
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 225 kDa (rat); antigen ~150 kDa (human)
• 交差性: rat, human, chicken
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.0 mg/mL
• テクニック: immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded rat heart.; indirect immunofluorescence: 3-6 μg/mL using chicken embryo fibroblast cells.; western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human tensin 1
• UniProtアクセッション番号: Q9HBL0
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... TNS1(7145) ; mouse ... Tns1(21961) ; rat ... Tns1(301509)

詳細

詳細

Tensin 1 (TNS1) is encoded by the gene mapped to human chromosome 2q35. The encoded protein is localized to focal adhesions and is mainly expressed in renal tubular epithelial cells (TECs).

免疫原

synthetic peptide corresponding to a sequence located in the internal region of human tensin1, conjugated to KLH. The corresponding sequence is identical in rat and mouse tensin1.

アプリケーション

Anti-Tensin 1 antibody may be used in immunofluorescence and immunohistochemistry.

Anti-Tensin 1 antibody produced in rabbit has been used in immunostaining and immunoblotting.

生物化学的／生理学的作用

Tensin 1 (TNS1) contributes to transforming growth factor (TGF)-β-induced myofibroblast differentiation and extracellular fibronectin and collagen matrix formation. Disruption in the gene and associated signaling might hinder tissue fibrosis. The encoded protein anchors to stress fibers by interacting with cytoplasmic tails of integrins and increases metastatic abilities of cancer cells. Tensin plays a vital role in activation of the c-Jun N-terminal kinases (JNK) and p38 pathways.

Tensin 1 associates with actin, integrin, FAK, p130Cas, DLC-1 and protein phosphatase PP1 which affects cell polarization, migration and invasion. Tensin 1 undergoes extensive Ser/Thr and Tyr phosphorylation induced by extracellular matrix (ECM), various growth factors and oncogenes. In addition, tensin 1 is cleaved by calpain II, a focal adhesion protease, suggesting that tensin 1 plays important roles in organizing the actin cytoskeleton and mediating signal transduction.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable