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Merck

PIR00

Sigma-Aldrich

Inhibitor Resistant Genotyping PCR ReadyMix

for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems

別名:

Inhibitor Resistant Genotyping PCR ReadyMix

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About This Item

UNSPSCコード:
41106300
NACRES:
NA.55

品質水準

形状

liquid

使用法

sufficient for 1250 reactions
sufficient for 250 reactions
sufficient for 5000 reactions

特徴

Difficult Templates/Specialty Enzymes PCR
dNTPs included
hotstart

保管条件

protect from light

テクニック

qPCR: suitable

light blue

入力

crude DNA

適合性

Bio-Rad CFX384
Bio-Rad CFX96
Bio-Rad MJ Chromo4
Bio-Rad MJ Opticon 2
Bio-Rad MJ Opticon
Bio-Rad MiniOpticon
Cepheid SmartCycler
Eppendorf® Mastercycler ep realplex
Eppendorf® Mastercycler ep realplex2 S
Illumina Eco qPCR
Qiagen Corbett Rotor-Gene 3000
Qiagen Corbett Rotor-Gene 6000
Qiagen Corbett Rotor-Gene Q
Roche LightCycler 480

検出方法

probe-based

輸送温度

dry ice

保管温度

−20°C

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関連するカテゴリー

詳細

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.

A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

アプリケーション

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix does not contain an internal reference dye.
Inhibitor Resistant Genotyping PCR ReadyMix has been used as a component of the reaction mixture for detection of leucoanthocyanidin reductase(PaLAR3) alleles in a genotyping assay and multiplex PCR for amplification of microsatellite.

特徴および利点

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Little/no optimization required
  • Compatible with both fast and conventional PCR cycling protocols

構成

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

その他情報

Compatible instruments include:
  • Bio-Rad® CFX384
  • Bio-Rad CFX96
  • Bio-Rad MiniOpticon
  • Bio-Rad/MJ Chromo4
  • Bio-Rad/MJ Opticon 2
  • Bio-Rad/MJ Opticon
  • Cepheid SmartCycler®
  • Eppendorf Mastercycler® ep realplex
  • Eppendorf Mastercycler ep realplex2 s
  • Illumina Eco qPCR
  • Qiagen/Corbett Rotor-Gene® 3000
  • Qiagen/Corbett Rotor-Gene 6000
  • Qiagen/Corbett Rotor-Gene Q
  • Roche LightCycler® 480

法的情報

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf AG
MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SmartCycler is a registered trademark of Cepheid, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

PIR00-1250RXN:
PIR00-5000RXN-PW:
PIR00-1250RXN-PW:
PIR00-5000RXN:
PIR00-250RXN-PW:
PIR00-SAMPLE-PW:
PIR00-250RXN:
PIR00-SAMPLE:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Root Rot Resistance Locus PaLAR3 Is Delivered by Somatic Embryogenesis (SE) Pipeline in Norway Spruce (Picea abies (L.) Karst.)
Edesi J, et al.
Forests, 12(2), 193-193 (2021)

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