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詳細
for use with Life Technologies XCell SureLock™ XCell I™, XCell II™ Mini-Cell and Bolt™ Mini Gel Tank
シェルフライフ
24 mo. at 2‑8 °C (from the date of manufacture)
包装
box of 10 ea
濃度
4-20% polyacrylamide
カセットW × L
10 cm × 10 cm
ゲル厚
1 mm
ウェルサイズ × 容積
12 wells × 35 μL
適合性
for use with Lonza Ltd PAGEr™ Minigel Chamber
保管温度
2-8°C
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アプリケーション
TruPAGE™ Tris-MOPS SDS Express Running Buffer has been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
特徴および利点
TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The precast polyacrylamide gels for SDS-PAGE come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.
There is no tape to remove. There is also no comb to remove, which prevents torn or bent wells. Instead, the cassettes lock well fingers in place.
The precast polyacrylamide gels for SDS-PAGE come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.
- Long Shelf Life - 2 years from the date of manufacture when stored properly.
- High Performance - Extended full length resoling gels provides improved resolution.
- Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
- Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
- Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
- Fast - Run times as low as 30 minutes.
- Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.
There is no tape to remove. There is also no comb to remove, which prevents torn or bent wells. Instead, the cassettes lock well fingers in place.
法的情報
Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
PAGEr is a trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
毒物及び劇物取締法
劇物
Jan Code
PCG2004-10EA:4548174010402
PCG2004-2EA:4548174010419
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Detection of phosphorylated Akt and MAPK in cell culture assays.
Molgaard S, et al.
MethodsX, 3, 386-398 (2016)
Simon Molgaard et al.
MethodsX, 3, 386-398 (2016-06-09)
This article describes an immunocytochemistry (ICC) method for staining against phosphorylated forms of the kinases Akt (pAkt) and MAPK (pMAPK). Phosphorylation is induced upon their activation by a number stimuli including insulin and brain-derived neurotrophic factor (BDNF), and is prerequisite
Ashraful Islam et al.
BMC musculoskeletal disorders, 20(1), 19-19 (2019-01-12)
Autologous chondrocyte implantation (ACI) has been used over the last two decades to treat focal cartilage lesions aiming to delay or prevent the onset of osteoarthritis; however, some patients do not respond adequately to the procedure. A number of biomarkers
Jae Myoung Suh et al.
Nature, 513(7518), 436-439 (2014-07-22)
Fibroblast growth factor 1 (FGF1) is an autocrine/paracrine regulator whose binding to heparan sulphate proteoglycans effectively precludes its circulation. Although FGF1 is known as a mitogenic factor, FGF1 knockout mice develop insulin resistance when stressed by a high-fat diet, suggesting a
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