P7965

モノクロナール抗P-糖タンパク質(MDR) マウス宿主抗体

clone F4, ascites fluid

• 別名: Anti-ABC20, Anti-CD243, Anti-CLCS, Anti-GP170, Anti-MDR1, Anti-P-GP, Anti-PGY1, Anti-p-170
• MDL番号: MFCD00282355
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: F4, monoclonal
• 分子量: antigen 170-180 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: hamster, human
• テクニック: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human kidney sections; immunohistochemistry (frozen sections): suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; radioimmunoassay: suitable using cell-surface RIA; western blot: suitable
• アイソタイプ: IgG1
• UniProtアクセッション番号: P08183
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... ABCB1(5243)

詳細

詳細

The ABCB1 (ATP binding cassette subfamily B member 1) gene is mapped to human chromosome 7q21.12. It encodes for P-glycoprotein, which is a membrane bound drug transporter protein, belonging to the ATP-binding cassette (ABC) transporters family. The gene is considered to be highly polymorphic.

特異性

本抗体は、P-糖タンパク質 (Pgp)の3番目の細胞外ル-プのN末端側半分にあるエピト-プを認識します。このエピト-プは、ホルマリン固定および過ヨウ素酸酸化に対して安定です。本抗体は、ヒト MDR1 P-糖タンパク質を特異的に検出しますが、ヒト MDR3、<REFERENCE ID="16647" TYPE="literature″/>マウスmdr1a、 mdr1b、mdr3 P-糖タンパク質<REFERENCE ID="16648" TYPE="literature″/>は認識しません。

免疫原

ヒトおよびハムスタ-の薬剤耐性全細胞および未精製の細胞膜の混合物です。

アプリケーション

Monoclonal Anti-P-Glycoprotein (MDR) antibody produced in mouse has been used in western blot analysis and Immunohistochemistry.

生物化学的／生理学的作用

Overexpression of ABCB1 (ATP binding cassette subfamily B member 1) gene is a major cause for multi-drug resistance, which makes chemotherapy challenging for the treatment of osteosarcoma. P-glycoprotein (P-gp) mediates the energy-dependent efflux of xenobiotic to the outside of plasma membrane from the inside of the cell , thereby affecting the process of absorption, distribution, and excretion of drugs. Cyclosporine, a calcineurin inhibitor serves as a substrate for P-gp.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable