P3326

血小板由来成長因子-AB ヒト

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 別名: PDGF-AB
• MDL番号: MFCD00213024
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• product name: 血小板由来成長因子-AB ヒト, PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 由来生物: human
• 品質水準: 200
• リコンビナント: expressed in E. coli
• アッセイ: ≥97% (SDS-PAGE and N-terminal analysis)
• 形状: lyophilized powder
• 有効性: 10-100 ng/mL ED₅₀/EC₅₀
• 品質: endotoxin tested
• 分子量: 27-31 kDa
• 包装: pkg of 10 μg; pkg of 50 μg
• 保管条件: avoid repeated freeze/thaw cycles
• テクニック: cell culture | mammalian: suitable
• 不純物: ≤1 EU/μg
• UniProtアクセッション番号: P04085, P01127
• 保管温度: −20°C
• 遺伝子情報: human ... PDGFA(5154) , PDGFB(5155)

詳細

詳細

Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.

アプリケーション

Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.

生物化学的／生理学的作用

Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.

その他情報

PDGFのA鎖とB鎖とのジスルフィド結合によるヘテロ二量体

物理的形状

凍結乾燥品(40%アセトニトリル、0.1%トリフルオロ酢酸溶液、0.2 μmフィルター濾過済み)。

アナリシスノート

生物活性は、休止期のNR6R-3T-3線維芽細胞の³H-thymidine取り込みに対する促進活性として測定されています。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)