O6014

Anti-O-GlcNAc Transferase (TI-14) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-O-linked N-Acetylglucosamine Transferase, 抗OGT抗体
• MDL番号: MFCD07370422
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 110 kDa
• 化学種の反応性: mouse, human, rat
• テクニック: indirect immunofluorescence: 1:50-1:100 using A549 human lung carcinoma cells fixed with paraformaldehyde/triton; western blot: 1:1,000-1:2,000 using HeLa cell nuclear extracts
• UniProtアクセッション番号: O15294
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... OGT(8473) ; mouse ... Ogt(108155) ; rat ... Ogt(26295)

詳細

詳細

O-GlcNAc transferase (OGT) catalyzes the addition of an N-acetylglucosamine residue to the amino acids serine or threonine. The enzyme is an homotrimer consisting of three subunits of 110 kDa each, with multiple tetratricopeptide (TPR) repeats.

免疫原

synthetic peptide corresponding to amino acids 1024-1037 of human O-GlcNAc transferase, conjugated to KLH via an N-terminal added cysteine residue.

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

生物化学的／生理学的作用

O-linked β-N-acetyl glycosamine (O-GlcNAc) is involved in repressing transcription. O-GlcNAc transferase (OGT) interacts with a histone deacetylase complex by binding to the co repressor Sin3A. This interaction leads to the repression of transcription, after transcription factors and RNA polymerase II are modified by addition of O-GlcNAc. O-GlcNAc modification reversibly inhibits proteosomal function in an ubiquitin-independent fashion.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)