MAK561

Succinate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

• 別名: SDH Activity Assay Kit
• UNSPSCコード: 41116104
• NACRES: NA.28

特性

• 使用法: sufficient for 100 colorimetric tests
• 品質水準: 100
• 検出方法: colorimetric
• 関連疾患: neurological disorders
• 保管温度: −20°C

詳細

詳細

Succinate Dehydrogenase (SDH) is part of complex II found in the inner part of the mitochondrial membrane. SDH is part of the citric acid cycle (TCA) that catalyze the oxidation of succinate to fumarate. Electrons from succinate are transferred to FAD forming FADH₂ which transfer electrons to CoQ. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome. The Succinate Dehydrogenase Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

アプリケーション

Succinate Dehydrogenase Assay Kit has been used to measure the SDH enzyme activity in cell and tissue culture supernatants and in purified mitchondria.

特徴および利点

• Highly sensitive, simple and high-throughput assay for measuring Succinate Dehydrogenase (SDH) concentration.
• Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.

Click here to download a calculator excel file

適合性

Suitable for the detection of Succinate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

安全性情報

• ピクトグラム: GHS06,GHS09
• シグナルワード: Danger
• 危険有害性情報: H302,H311 + H331,H411
• 注意書き: P261 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P311
• 危険有害性の分類: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2
• 保管分類コード: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• 引火点(°F): 188.6 °F
• 引火点(℃): 87 °C