MAK452

Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit

Sufficient for 100 colorimetric tests

• 別名: G6PDH Inhibitor Screening Kit
• UNSPSCコード: 12161503
• NACRES: NA.54

特性

• 品質水準: 100
• 検出方法: colorimetric
• 関連疾患: hematological disorder, anemia; cancer
• 保管温度: −20°C

詳細

詳細

Glucose-6-Phosphate Dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.

アプリケーション

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit may be used for:

• Cancer Research
• Drug Discovery

特徴および利点

• Efficient Inhibitor Screening: Our kit allows for efficient screening of G6PDH inhibitors using just 5 μL of test compound, minimizing effort while ensuring accurate results.
• Compatibility with High-Throughput Systems: Seamlessly integrating into high-throughput handling systems, our kit guarantees efficiency and accuracy, streamlining your screening processes.

適合性

The kit is suitable for high throughput inhibitor screening and evaluation of glucose-6-phosphate dehydrogenase inhibitors.

原理

The Glucose-6-Phosphate Dehydrogenase Inhibitor Screening Kit is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at A₅₆₅ nm. The increase in absorbance at A₅₆₅ nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

その他情報

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安全性情報

• ピクトグラム: GHS08
• シグナルワード: Warning
• 危険有害性情報: H341
• 注意書き: P201 - P202 - P280 - P308 + P313 - P405 - P501
• 危険有害性の分類: Muta. 2
• 保管分類コード: 12 - Non Combustible Liquids