MAK166

Fluorimetric Hydrogen Peroxide Assay Kit

sufficient for 500 fluorometric tests (infrared fluorescence)

• 別名: Hydrogen Peroxide Detection Kit
• EC Number: 231-765-0
• UNSPSCコード: 12161503
• NACRES: NA.84

特性

• 使用法: sufficient for 500 fluorometric tests (infrared fluorescence)
• 検出方法: fluorometric
• 関連疾患: orthopedic diseases; cardiovascular diseases; aging/geriatric diseases; neurological disorders; pulmonary disorders
• 保管温度: −20°C

詳細

詳細

Hydrogen peroxide, a reactive oxygen species produced through the metabolism of molecular oxygen, serves as both an intracellular signaling messenger and a source of oxidative stress. Hydrogen peroxide is generated in cells via multiple mechanisms such as the NOX-mediated ROS production by neutrophils and macrophages (respiratory burst) or by the dismutase of superoxide anions produced as a result of electron leak during mitochondrial respiration. Abnormal hydrogen peroxide production contributes to oxidative cell damage and the progression of diseases such as asthma, atherosclerosis, osteoporosis, and neurodegeneration.

アプリケーション

Fluorimetric Hydrogen Peroxide Assay Kit has been sued to determine the oxidation pressure (H₂O₂).

特徴および利点

This assay is compatible with high-throughput handling systems.

適合性

The Fluorescent Hydrogen Peroxide Assay Kit provides a simple and reproducible method to quantify hydrogen peroxide levels in a variety of samples such as cellular extracts and solutions.

原理

Fluorimetric Hydrogen Peroxide Assay Kit can also be used to detect hydrogen peroxide released from living cells or produced by oxidase activities. This kit utilizes a peroxidase substrate that generates an infrared fluorescent product (λ_ex = 640/λ_em = 680 nm) after reaction with hydrogen peroxide that can be analyzed by a fluorescent microplate reader. The generation of the fluorescence product is pH independent between pH 4 to pH 10 and is superior to other fluorescence kits for measuring hydrogen peroxide in low pH solutions which often display decreased probe sensitivity. The infrared fluorescent probe is less sensitive to assay background due to autofluorescence from biological samples. This assay is compatible with high-throughput handling systems.

安全性情報

• ピクトグラム: GHS08
• シグナルワード: Danger
• 危険有害性情報: H334
• 注意書き: P261 - P284 - P501
• 危険有害性の分類: Resp. Sens. 1
• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable