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使用法
sufficient for 500 fluorometric tests
検出方法
fluorometric
保管温度
−20°C
遺伝子情報
human ... ACP1(52) , ACP2(53)
mouse ... ACP1(11431) , ACP2(11432)
rat ... ACP1(24161) , ACP2(24162)
詳細
The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.
特徴および利点
Compatible with high-throughput handling systems.
適合性
Suitable for the detection of acid phosphatase in serum and other biological samples.
原理
The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.
保管分類コード
12 - Non Combustible Liquids
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
MAK087-1KT:
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試験成績書（COA）
Lot/Batch Number
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Significance of increased phosphatase activity of bone at the site of osteoplastic metastases secondary to carcinoma of the prostate gland.
Gutman E B, et al.
Cancer Research, 28(3), 485-495 (1936)
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Plant physiology, 151(1), 199-209 (2009-07-28)
Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with
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