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由来生物
mouse
品質水準
結合体
unconjugated
抗体製品の状態
purified from hybridoma cell culture
抗体製品タイプ
primary antibodies
クローン
ERK-NP2, monoclonal
形状
buffered aqueous solution
分子量
antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa
化学種の反応性
human, rat
濃度
~2 mg/mL
テクニック
immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-25 μg/mL using rat brain extract
アイソタイプ
IgG1
UniProtアクセッション番号
輸送温度
dry ice
保管温度
−20°C
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... MAPK1(5594) , MAPK3(5595)
rat ... Mapk1(116590) , Mapk3(50689)
詳細
Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.
特異性
本抗体は、リン酸化されていない非活性化型のMAPキナ-ゼ(ERK-1およびERK-2)と特異的に反応します。一リン酸化型(スレオニンまたはチロシン)とわずかな交差反応が観察されますが、MAPKの二リン酸化型ペプチドとは反応しません。抗体とJNKまたはp38 MAPKとの反応は認められません。エピト-プには、ERK活性化ル-プ(ERK-2の場合178-188)に存在する非リン酸化型のスレオニン183およびチロシン185が含まれています。
免疫原
非リン酸化型のERK活性化ル-プに相当する合成ペプチド(HTGFLTEYVAT)。
アプリケーション
A working concentration of 5-25 μg/mL may be used for detection by immunoblotting in rat brain extract. The antibody has also been used at working dilution of 1:50 for MAPK quantification by flow cytometry in monocyte-derived macrophages (MDM) derived from Holstein cattle.
物理的形状
PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。
調製ノート
バイオリアクタ-で増殖させたハイブリド-マの培養上清から調製しています。
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
10 - Combustible liquids
WGK
nwg
引火点(°F)
Not applicable
引火点(℃)
Not applicable
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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