M7927

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL番号: MFCD01633615
• UNSPSCコード: 12352203
• NACRES: NA.44

特性

• 由来生物: rabbit
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen, ERK-1 44 kDa; antigen, ERK-2 42 kDa
• 化学種の反応性: mouse, rat, human
• テクニック: immunoprecipitation (IP): 5 μg using NIH 3T3 mouse fibroblast lysates; microarray: suitable; western blot: 1:5,000 using NIH 3T3 mouse fibroblast lysate or rat brain extract
• UniProtアクセッション番号: P27361
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MAPK3(5595) ; mouse ... Mapk3(26417) ; rat ... Mapk3(50689)

詳細

詳細

Mitogen-activated protein kinase 3 (ERK1) is mapped to human chromosome 16p11.2. It has a serine/threonine domain and a transactivation domain (TAD) and a nuclear localization sequence (NLS).

特異性

By immunoblotting, the antibody reacts with ERK-1 and ERK-2.

免疫原

synthetic peptide corresponding to amino acids 351-368 of human ERK-1.

アプリケーション

Anti-MAP Kinase (ERK-1, 351-368) antibody produced in rabbit has been used in:

• immunofluorescence
• immunoprecipitation
• western blotting.

生物化学的／生理学的作用

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), c-Jun N-terminal kinases (JNK) and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK1 (p44) and ERK2 (p42) require the dual phosphorylation in the catalytic kinase domain by MEKs for their full activity. ERK1 is phosphorylated on Tyr204 and Thr202, and ERK2 on Tyr187 and Thr185. ERK1 and 2 may also undergo autophosphorylation on these residues. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)