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product name
イーグル最少必須培地, Modified, with Earle′s salts and reduced NaHCO3. without L-glutamine, liquid, sterile-filtered, suitable for cell culture
品質水準
無菌性
sterile-filtered
形状
liquid
テクニック
cell culture | mammalian: suitable
不純物
endotoxin, tested
構成
phenol red: 0.011 g/L
NaHCO3: 0.85 g/L
glucose: 1.0 g/L
L-glutamine: no
輸送温度
ambient
保管温度
2-8°C
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詳細
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
アプリケーション
二酸化炭素を使用しない培養系に推奨されます。
その他情報
本製品にはL-Ala、L-Asn、L-Glu、Gly、L-Pro、L-Ser、L-Glnが含まれていません。
緩衝範囲を広げるためにHEPESが補充されることもあります。
再構成
0.292 g/LのL-グルタミンを添加してください。
よく一緒に購入される製品
製品番号
詳細
価格
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
M2414-BULK:
M2414-6X500ML:
M2414-VAR:
M2414-500ML:
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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