FG0325

Minimum Essential Medium Eagle

With Earle′s salts, stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

• 別名: EMEM, MEM
• UNSPSCコード: 12352207
• NACRES: NA.71

特性

• product name: Minimum Essential Medium Eagle, With Earle′s salts, L-alanyl-glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
• 品質水準: 400
• 無菌性: sterile-filtered; sterile
• 形状: liquid
• テクニック: cell culture | mammalian: suitable
• 不純物: Endotoxin, tested
• 構成: stable glutamine: yes; L-glutamine: no; phenol red: yes; sodium pyruvate: no; Earle’s salts (5% CO₂): yes; HEPES: no; Hanks’ salts (2% CO₂): no; glucose: yes; NaHCO₃: yes
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

アプリケーション

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

その他情報

This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 1
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable