I5271

IL-1β mouse

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 別名: IL-1β, リンパ球活性化因子
• MDL番号: MFCD00164815
• UNSPSCコード: 12352202
• NACRES: NA.77

特性

• 製品名: インターロイキン-1β マウス由来, IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 由来生物: mouse
• 品質水準: 200
• リコンビナント: expressed in E. coli
• アッセイ: ≥97% (SDS-PAGE)
• フォーム: lyophilized powder
• 有効性: ≤0.010 ng/mL ED₅₀
• 品質: endotoxin tested
• 分子量: 17.5 kDa (containing 153 amino acid residues)
• 包装: pkg of 5X5 μg; pkg of 5 μg
• テクニック: cell culture | mammalian: suitable
• 不純物: ≤0.1ng/mg
• UniProtアクセッション番号: P10749
• 保管温度: −20°C
• 遺伝子情報: mouse ... Il1b(16176)

詳細

詳細

Interleukin-1β (IL-1β) cytokine is generated by monocytes, dendritic cells, macrophages and keratinocytes. Pro-IL-1β, an IL-1β precursor undergoes proteolytic cleavage to produce its active form of 17 kDa.

アプリケーション

Interleukin-1β from mouse has been used:

• as a proinflammatory agent, to induce inflammatory signaling cascades mediated cellular damage
• to study its effect on fetuin A-induced inflammatory response in podocyte
• to check its effect on the expression of mitogen-activated protein kinase 4 using 3T3-L1 adipocytes

生物化学的／生理学的作用

Interleukin-1β (IL-1β) is a potent inflammatory cytokine, and mediates inflammation through proinflammatory molecules: cyclooxygenase-2 (COX2) and nitric oxide. IL-1β release is stimulated by injury and increased levels are observed in response to severe pain. It has its main function in T-cell activation, antigen recognition and T-helper cell 17 cell development. IL-1β is also responsible for the formation of IL-17 and IL-22 by nature killer T (NKT) cells and NK cells. It is known to induce inflammation in psoriasis and rheumatoid arthritis. IL-1β controls intercellular adhesion molecule-1 expression. It is significantly associated with bone damage and vascular injury.

物理的形状

凍結乾燥品(0.2 μmフィルターで滅菌濾過済み、10 mMクエン酸ナトリウム、pH 4.0を含有)。

アナリシスノート

生物活性は、マウスD10S細胞の用量依存性刺激を指標として測定しました。

その他情報

インターロイキン1α(IL-1α)とインターロイキン1B(IL-1Β)という2つの密接に関連する物質は、同じ細胞表面受容体に結合し、ほぼ同一の生物学的反応を誘発しますが、アミノ酸配列の相同性は25%です。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable