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製品名
シスタチンC ヒト, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
由来生物
human
品質水準
リコンビナント
expressed in HEK 293 cells
アッセイ
≥95% (SDS-PAGE)
フォーム
lyophilized powder
有効性
≤5 μM IC50
品質
endotoxin tested
分子量
dimer 12-13 kDa (non-glycosylated)
包装
pkg of 10 μg
保管条件
avoid repeated freeze/thaw cycles
テクニック
cell culture | mammalian: suitable
不純物
≤1 EU/mg
UniProtアクセッション番号
保管温度
−20°C
遺伝子情報
human ... CYTC(1471)
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詳細
HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.
アプリケーション
Cystatin C human has been used as a standard in western blot assay. It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).
生物化学的／生理学的作用
Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.
物理的形状
Lyophilized from a 0.2 μm filtered solution of 1×PBS
アナリシスノート
パパインのプロテアーゼ活性に対するシスタチンCの阻害機能は、基質としてL-BAPAを用いた比色分析アッセイによって測定されました。IC50値は、0.55 μMパパインと0.44 μM L-BAPAの存在下で1.56～50 μg/mLのシスタチンCを用いて5～20 μg/mL(0.3～1.5 μM)で測定されました。
法的情報
HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
H5041-BULK:
H5041-VAR:
H5041-10UG:
H5041-10UG-PW:
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Jean-Charles Lafarge et al.
Biochimie, 92(11), 1580-1586 (2010-04-27)
Given the increasing prevalence of human obesity worldwide, there is an urgent need for a better understanding of the molecular mechanisms linking obesity to metabolic and cardiovascular diseases. Our knowledge is nevertheless limited regarding molecules linking adipose tissue to downstream
Assessment of glomerular filtration rate in acute and chronic settings
National Kidney Foundation Primer on Kidney Diseases, 26-32 (2014)
Can-E Tang et al.
Journal of proteome research, 9(12), 6101-6111 (2010-10-05)
The epidermal growth factor receptor (EGFR) is usually overexpressed in nasopharyngeal carcinoma (NPC) and is associated with pathogenesis of NPC. However, while EGFR-modulated intracellular proteins have been extensively studied, little is known concerning their extracellular counterparts. To identify EGFR-regulated secreted
Hana Dvořáková et al.
Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)
Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate
Hyun Jung Chung et al.
PloS one, 10(7), e0133417-e0133417 (2015-07-18)
The high incidence of acute and chronic kidney injury due to various environmental factors such as heavy metals or chemicals has been a major problem in developing countries. However, the diagnosis of kidney injury in these areas can be more
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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