H0163

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal

clone HDAC4-144, purified from hybridoma cell culture

• MDL番号: MFCD05665442
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: HDAC4-144, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~140 kDa
• 交差性: human, rat, mouse
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• 濃度: ~2 mg/mL
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using total cell extracts of NIH3T3 fibroblasts cells
• アイソタイプ: IgG2a
• UniProtアクセッション番号: P56524
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... HDAC4(9759) ; mouse ... Hdac4(208727) ; rat ... Hdac4(363287)

詳細

詳細

Monoclonal Anti-Histone Deacetylase 4 (HDAC4) (mouse IgG2a isotype) is derived from the HDAC4-144 hybridoma produced by the fusion of mouse myeloma cells (NS1) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids of human HDAC4 with C-terminal added lysine, conjugated to KLH. HDAC4 belongs to the class II of Mammalian HDACs.

アプリケーション

Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• immunoprecipitation

生物化学的／生理学的作用

Histone deacetylase (HDAC) catalyzes the deacetylation of histones. HDAC4 is implicated in transcriptional repression. It dynamically migrates between nucleus and cytoplasm through its nuclear import and export signals. Interaction of HDAC4 with 14-3-3 and myocyte enhancer factor-2 (Mef2) proteins disturbs such shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable