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特徴
wetted part: no
包装
pkg of 50 ea (columns)
メーカー/製品名
Cytiva 28-9180-08
保管条件
dry media
ベッドサイズ
13 mm × 26 mm
ベッド容積
3.5 mL
カラム内径
13 mm
Matrix
cross-linked dextran
粒径
>50 μm (dry)
85-260 μm
cleaning
2-13
動作範囲
2-13
適合性
suitable for bioprocess medium
関連するカテゴリー
詳細
PD MidiTrap G-25 is a convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel. Can be used in a centrifugation or gravity protocol.
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.
アプリケーション
Protein sample preparation. Desalting/Buffer Exchange/Cleanup.
特徴および利点
- Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
- Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
- High desalting capacity.
- Flexible protocols with ability to use gravity flow or centrifugation.
- Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)
シグナルワード
Warning
危険有害性情報
保管分類コード
12 - Non Combustible Liquids
WGK
WGK 2
最新バージョンのいずれかを選択してください:
資料
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.
プロトコル
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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