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詳細
Escherichia coli strain B is most widely studied DNA after k-12. The genomes of E coli strain B and k-12 show differences in the IS sequence elements. Strain B have B-islands and express O-specific side-chain LPS. The product is unsheared genomic DNA.
特異性
50%GC;Tm = 90.5°C
アプリケーション
The DNAs are particularly useful for genomic analysis, including PCR, library construction in bacteriophage λ, and in heterologous pre-hybridization and hybridization protocols.
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used for:
- PCR reactions of genomic DNA of Gardnerella vaginalis
- nick translation and radiolabeling of dNTP
- generating standard curve for 16S rDNA quantification
- transfecting neutrophils
- testing the extraction methods
単位の定義
One unit will yield an A260 of 1.0 in 1.0 ml of 1 mM Tris-HCl, pH 7.5, with 1 mM NaCl and 1 mM EDTA (1 cm light path).
調製ノート
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of approx. 2 units per mL. Approx. 20 A260 units per mg DNA. % GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
アナリシスノート
DNAの質・完全性の評価は、パルスフィ-ルドゲル電気泳動およびエチジウムブロマイド染色により行っています。DNAには、RNAや制限酵素消化を阻害するおそれのある不純物は含まれません。
その他情報
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
関連製品
製品番号
詳細
価格
保管分類コード
11 - Combustible Solids
WGK
WGK 3
引火点(°F)
Not applicable
引火点(℃)
Not applicable
個人用保護具 (PPE)
Eyeshields, Gloves, type N95 (US)
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
D4889-5UN:
D4889-BULK-N:
D4889-.25UN:
D4889-PH:
D4889-VAR:
D4889-BULK:
D4889-1UN:
D4889-.25UN-PW:
D4889-1UN-PW:
D4889PROC:
D4889-5UN-PW:
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