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Merck

GE28-9031-33

Protein A High Performance MultiTrap

Cytiva 28-9031-33

別名:

High Performance Protein A, MultiTrap Protein A

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About This Item

UNSPSCコード:
41105323
NACRES:
NA.25

ligand

native protein A

物質

polypropylene and polyethylene

特徴

wetted part: no

包装

pkg of 4 ea

メーカー/製品名

Cytiva 28-9031-33

保管条件

(20% Ethanol)

パラメーター

30 °C max. temp.
4 °C min. temp.

サイズ

127.8 mm × 85.5 mm × 30.6 mm

Matrix

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

2-10

動作範囲

3-9

保管温度

2-8°C

関連するカテゴリー

詳細

Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species.

Protein A Sepharose High Performance has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen (protein of interest) to be eluted separately (cross-link protocol) or together with the antibody (classic protocol). Remember to order the collection plates for washing and elution of the enriched protein.

There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

特徴および利点

  • Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
  • Flexible format, with protocols for classic elution and cross-link method.
  • Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Easy scale-up with HiTrap® Protein A HP prepacked columns.
  • Prepacked with Protein A Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • MultiTrap 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page describes immunoprecipitation (immunoaffinity or pull-down techniques) with GE Healthcare products.

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