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ligand
glutathione
物質
polypropylene and polyethylene
メーカー/製品名
Cytiva 28-4055-00
包装
pkg of 4 x 96-well
サイズ
127.8 mm × 85.5 mm × 30.6 mm
Matrix
4% agarose
粒径
45-165 μm
平均直径
90 μm
cleaning
4-13
動作範囲
4-13
キャパシティ
>25 mg binding capacity (horse liver GST/ml medium)
詳細
アプリケーション
Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap™ filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.
GST MultiTrap™ FF is prepacked with Glutathione Sepharose™ 4 Fast Flow, while GST MultiTrap™ 4B is prepacked with Glutathione Sepharose™ 4B.
特徴および利点
- 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
- Highly reproducible well-to well and plate-to-plate results.
- High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
- Wells are prepacked with Glutathione Sepharose™ media for convenience.
- Easy and predictable scale-up using prepacked GSTrap™ FF, GSTPrep™ FF 16/10, and GSTrap™ 4B columns.
保管および安定性
アナリシスノート
法的情報
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
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資料
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.
This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.
This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.
プロトコル
This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.
This page shows how to solubilize membrane proteins with products from GE Healthcare.
Characteristics of Glutathione Sepharose® Products
Characteristics of Glutathione Sepharose® Products
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