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Merck

GE28-4055-00

GST MultiTrap 4B

Cytiva 28-4055-00

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About This Item

UNSPSCコード:
12161501
NACRES:
NA.32

ligand

glutathione

物質

polypropylene and polyethylene

メーカー/製品名

Cytiva 28-4055-00

包装

pkg of 4 x 96-well

サイズ

127.8 mm × 85.5 mm × 30.6 mm

Matrix

4% agarose

粒径

45-165 μm

平均直径

90 μm

cleaning

4-13

動作範囲

4-13

キャパシティ

>25 mg binding capacity (horse liver GST/ml medium)

詳細

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

アプリケーション

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap FF is prepacked with Glutathione Sepharose 4 Fast Flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.

特徴および利点

  • 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
  • Highly reproducible well-to well and plate-to-plate results.
  • High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
  • Wells are prepacked with Glutathione Sepharose media for convenience.
  • Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

GSTPrep is a trademark of Cytiva
GSTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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資料

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

プロトコル

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

This page shows how to solubilize membrane proteins with products from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

Characteristics of Glutathione Sepharose® Products

すべて表示

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