包装
pack of 5 mL
メーカー/製品名
Cytiva 17-5318-06
マトリックス
6% cross-linked agarose
粒径
45-165 μm
平均直径
90 μm
cleaning in place
2-14(Ni2+-stripped medium.)
working range
3-12(Ni2+-stripped medium.)
キャパシティ
~40 mg binding capacity(histidine-tagged protein)
適合性
suitable for bioprocess medium
詳細
アプリケーション
Ni Sepharose™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix and pre-charged with Ni2? ions for purification of histidine tagged Proteins.
The coupling chemistry and the novel ligand is designed to give minimal ligand and Ni2? leakage while providing high capacity.
Ni Sepharose™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Ni Sepharose™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.
特徴および利点
- Designed for scaling up purification of histidine-tagged Proteins
- High Protein binding capacity and minimal leakage of Ni2? ions
- As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
- Available in convenient prepacked HisPrep™ and HisTrap™ column formats.
保管および安定性
アナリシスノート
法的情報
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
シグナルワード
Warning
危険有害性情報
保管分類コード
3 - Flammable liquids
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
資料
This page shows high-throughput screening using His MultiTrap HP and His MultiTrap FF 96-well filter plates from GE Healthcare.
プロトコル
This page shows how to purify histidine-tagged recombinant proteins manually with a syringe using HisTrap FF crude Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged recombinant proteins by gravity flow using His GraviTrap and His GraviTrap Kit from GE Healthcare.
This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using HisTrap Excel from GE Healthcare.
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