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GE17-5143-01

HiTrap® Benzamidine Fast Flow

Cytiva 17-5143-01, pack of 5 × 1 mL

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

p-aminobenzamidine

包装

pack of 5 × 1 mL

メーカー／製品名

Cytiva 17-5143-01

保管条件

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

パラメーター

42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

マトリックス

highly cross-linked 4% agarose

平均直径

90 μm

cleaning

1-9

working range

2-8

保管温度

2-8°C

詳細

HiTrap™ Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose™ 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.

アプリケーション

HiTrap™ Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.

The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.

特徴および利点

  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
  • Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
  • HiTrap™ Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
  • High binding capacity.
  • The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA™ design or FPLC™ System.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

HiTrap is a registered trademark of Cytiva

ピクトグラム

Flame
GHS02

シグナルワード

Warning

危険有害性情報

H226

保管分類コード

3 - Flammable liquids

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

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資料

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

すべて表示

プロトコル

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

Removal of Thrombin and Factor Xa Using HiTrap Benzamidine FF (High Sub)

Performing a Separation with GE Healthcare Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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