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Key Documents

GE17-5110-01

HiTrap^® IGM purification

Name: HiTrap<SUP>®</SUP> IGM purification
Brand: SIGMA

Cytiva 17-5110-01, pack of 5 × 1 mL

別名:

IGM Purification Column

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About This Item

UNSPSCコード:

23151816

NACRES:

NA.56

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包装

pack of 5 × 1 mL

メーカー／製品名

Cytiva 17-5110-01

保管条件

(20% Ethanol)

パラメーター

<4 mL/min flow rate
42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

16 mm

マトリックス

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

2-13

working range

3-11

保管温度

2-8°C

詳細

HiTrap^® IgM Purification HP is a 1 mL column prepacked with Sepharose^™ High Performancefor fast and efficient purification of monoclonal IgM.

アプリケーション

HiTrap^® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

特徴および利点

Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose^™ High Performance.
Recovery of IgM is high with excellent activity retention.
The top and bottom frits are manufactured from porous polyethylene
Columns are delivered with a stopper on the inlet and a snap-off end on the outle

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.