GE17-3712-06

HisTrap^™ Excel

Cytiva 17-3712-06, pack of 5 × 5 mL

• 別名: affinity resins, Ni Sepharose excel column, ion affinity chromatography column, IMAC column
• UNSPSCコード: 23151816
• NACRES: NA.56

特性

• 形状: prepacked column
• クロスリンク: cross-linked
• 包装: pack of 5 × 5 mL
• メーカー／製品名: Cytiva 17-3712-06
• 保管条件: (20% Ethanol)
• パラメーター: 5 bar; 5 ml/min - 20 ml/min flow rate
• テクニック: liquid chromatography (LC): suitable
• ベッドサイズ: 16 mm × 25 mm
• ベッド容積: 5 mL
• カラム内径: 16 mm
• マトリックス: highly cross-linked 6% agarose
• マトリックス活性基: Nickel
• 平均直径: 90 μm
• cleaning: 2-14
• working range: 2-13
• キャパシティ: ≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)
• 分離法: affinity

詳細

詳細

Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.

HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC). HisTrap excel 1 mL and 5 mL are ready-to-use IMAC columns prepacked with Ni Sepharose excel. The design of the columns in combination with the specific properties of the resin enables fast and convenient purifications. The special type of filter in the top and bottom of the columns makes it possible to load large volumes of cell-free, unclarified samples directly on the columns without causing back pressure problems. This time-saving property helps prevent degradation and loss of sensitive target proteins.

アプリケーション

Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose^™ excel medium resistant to chelating and reducing agents

特徴および利点

• Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
• Increase target protein yield and decrease degradation through reduced and simplified sample handling
• HisTrap excel columns allow direct purification of cell-free, unclarified samples
• HisTrap excel columns are made of biocompatible polypropylene. The columns are delivered with a stopper on the inlet and a snap-off end on the outlet.
• HisTrap excel columns can be operated either with a peristaltic pump or with chromatography systems such as ÄKTA systems. The columns cannot be opened or refilled.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.

The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.

法的情報

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

安全性情報

• ピクトグラム: GHS09
• シグナルワード: Warning
• 危険有害性情報: H226
• 注意書き: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• 保管分類コード: 3 - Flammable liquids