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Merck

GE17-0921-07

IMAC Sepharose 6 Fast Flow

Cytiva 17-0921-07, pack of 25 mL

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About This Item

UNSPSCコード:
41106500
NACRES:
NA.56

包装

pack of 25 mL

メーカー/製品名

Cytiva 17-0921-07

Matrix

6% cross-linked agarose

粒径

45-165 μm

平均直径

90 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

動作範囲

3-12

キャパシティ

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

適合性

suitable for bioprocess medium

詳細

IMAC Sepharose 6 Fast FLow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

アプリケーション

IMAC Sepharose 6 Fast FLow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.


IMAC Sepharose 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.


The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.


IMAC Sepharose 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support

特徴および利点

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in the convenient and time-saving prepacked HiTrap® and HiPrep formats.

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

法的情報

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


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試験成績書(COA)

Lot/Batch Number

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資料

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

プロトコル

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

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製品に関するお問い合わせはこちら(テクニカルサービス)