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Key Documents

GE17-0408-01

HiTrap® Chelating High Performance

Cytiva 17-0408-01, pack of 5 × 1 mL

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About This Item

UNSPSCコード:
23151816
NACRES:
NA.56

ligand

iminodiacetic acid

包装

pack of 5 × 1 mL

メーカー/製品名

Cytiva 17-0408-01

パラメーター

<4 mL/min flow rate
42 psi

ベッドサイズ

7 mm × 25 mm

ベッド容積

1 mL

カラム内径

7 mm

マトリックス

highly cross-linked 6% agarose

平均部品サイズ

34 μm

cleaning

2-14

working range

3-13

詳細

HiTrap® Chelating HP is prepacked with Chelating Sepharose High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

アプリケーション

HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC System

特徴および利点

  • For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
  • Conveniently charge with your metal of choice.
  • Iminidoacetic acid coupled to Sepharose High Performance.
  • Purifying Proteins with exposed histidine groups via metal ion complex formation.
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC System.
  • Packed with Chelating Sepharose High Performance
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System

保管および安定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

アナリシスノート

この製品の分析証明書は、www.cytiva.com.

その他情報

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法的情報

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

ピクトグラム

Flame

シグナルワード

Warning

危険有害性情報

保管分類コード

3 - Flammable liquids


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

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資料

This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.

This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.

This page shows how to perform a purification and homogeneity check of membrane proteins with products from GE Healthcare.

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

プロトコル

This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

すべて表示

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