G9282

モノクロナール抗グルタメート マウス宿主抗体

clone GLU-4, ascites fluid

• MDL番号: MFCD00162331
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• クローン: GLU-4, monoclonal
• 含みます: 15 mM sodium azide
• 化学種の反応性: wide range
• テクニック: indirect ELISA: 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... GRM1(2911)

詳細

特異性

本抗体はグルタルアルデヒド固定サンプルにおいて、L-グルタミン酸 (L-グルタミン酸) と特異的に反応します。Gly-Glu, Asp-Gluとの交差反応が見られます。推奨される希釈倍率で間接/競合ELISAを行った場合、D-グルタミン酸, L-グルタミン酸, L-アスパラギン酸, D-アスパラギン酸, L-アスパラギン, β-アラニン, グリシン, 5-アミノ吉草酸, γ-アミノ酪酸 (GABA), Gly-Aspとの弱い交差反応がみられます。グルタミン酸は細胞体、軸索、およびCNSのグルタミン酸ニュ-ロン終末にみられます。

免疫原

L-グルタミン酸 (Glu)

アプリケーション

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immuno-electron microscopy (1 paper)

Monoclonal Anti-Glutamate antibody produced in mouse is suitable for:

• immunolabeling of glutamate in the lamina of the Drosophila visual system
• immunocytochemistry at a working dilution of 1:20,000 to detect glutamate using heart and extrinsic nerves of stomatopod crustacean, Squilla oratoria
• immunofluorescent staining as a primary antibody at a working dilution of 1:1000 using cryosections of brains and embryos from mice
• use as a primary antibody to measure the glutamate release in synaptosome preparations

It is also suitable for indirect ELISA at a working dilution of 1:10,000 using in situ prepared L-glutamate-glutaraldehyde-BSA conjugate for coating.

生物化学的／生理学的作用

Glutamate is an amino acid that acts as a excitatory neurotransmitter in the vertebrate brain and functions in all brain processes. It is released from one end of the neuron and it binds to receptors on the surface of adjacent neurons. Glutamate is released in a calcium-dependent manner in response to depolarizing stimuli. It serves as a substrate in certain metabolic pathways, such as, synthesis of glutamine and γ-aminobutyric acid (GABA) Excessive release of glutamate or aspartate have been observed in the pathogenesis of certain neurodegenerative diseases, such as, Huntington′s disease, Alzheimer′s disease. The same is also observed in selective neuronal degenerations involved in epilepsy, ischemia, and hypoglycemia.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable