G2404

モノクロナール抗ゴルジ58Kタンパク質/ホルムイミノトランスフェラーゼシクロデアミナーゼ (FTCD) マウス宿主抗体

ascites fluid

• 別名: Anti-LCHC1
• MDL番号: MFCD00164605
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: ascites fluid
• 抗体製品タイプ: primary antibodies
• 分子量: antigen 58 kDa
• 含みます: 15 mM sodium azide
• 化学種の反応性: human, bovine, mouse, pig, canine, kangaroo rat, hamster, monkey, rat
• テクニック: electron microscopy: suitable; indirect immunofluorescence: 1:50 using cultured CHO cells; western blot: 1:5,000 using whole rat liver extract
• アイソタイプ: IgG1
• UniProtアクセッション番号: O95954
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FTCD(10841) ; mouse ... Ftcd(14317) ; rat ... Ftcd(89833)

詳細

詳細

FTCD is a homooctamer with its subunits arranged in a planar ring. It is predominantly expressed in the liver. Apart from being associated with the Golgi apparatus, it is detectable in the supernatant cytosolic fraction, and may also be localized to cytoplasmic vesicles.

Monoclonal Anti-Golgi 58K Protein/formiminotransferase cyclodeaminase (FTCD) (mouse IgG1 isotype) is derived from the 58K-9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with FTCD/Golgi 58K protein purified from rat liver.

特異性

本抗体は、ゴルジ膜の微小管結合部位周辺 (the microtubule-binding peripheral) に存在する分子量 58 KDaのタンパク質上に位置するエピト-プを認識します。また、ゴルジ体に対する微小管撹乱因子 (microtubule-perturbing agent) の作用に関する研究にもご使用いただけます。

免疫原

ラット肝臓由来のゴルジ 58Kタンパク質です。

アプリケーション

Anti-Golgi FTCD antibody, clone 58k-9, Mouse monoclonal has been used in western blot analysis and dual immunofluorescence staining.

Anti-Golgi FTCD antibody, clone 58k-9, Mouse monoclonal is suitable for electron microscopy, indirect immunofluorescence at a working dilution of 1:50 using cultured CHO cells and indirect immunoblotting at 1:5000 working dilution using whole rat liver extract. It is also useful for the localization of Golgi 58K protein using immunoblotting, dot blot, electron microscopy, and immunocytochemistry.
The antibody was used:

• for the analysis of distribution of the 58K9 protein exclusively localized in the Golgi
• as a primary antibody in the immunofluorescence analysis in studies related to functioning of nuclear envelope protein TMEM209 in lung carcinoma cells, tracking TG2 (Transglutaminase Type 2) transport in renal tubular epithelial cells, binding of TRADD (TNFR-associated death domain protein) to TNF-R1 at the plasma membrane and localization of Wilson disease protein in the Golgi apparatus
• in immunoprecipitation studies

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)

生物化学的／生理学的作用

FTCD/58K protein of the Golgi apparatus used in conjunction with other antibodies to Golgi proteins (e.g., the Golgi β-COP protein) may be used for studies on the role and relationships of this protein in the cell. It is also useful for localization studies following subcellular fractionation procedures.

Golgi 58K protein is associated with the Golgi apparatus peripherally and has been identified as a version of FTCD (Formiminotransferase Cyclodeaminase). It is a bifunctional metabolic enzyme involved in conversion of histidine to glutamic acid. It acts as a channel for the transport of one-carbon units from formiminoglutamate to the folate pool. It also interacts with vimentin subunits and with polymerized vimentin filaments. Autosomal recessive disorder glutamate formiminotransferase deficiency is caused by defects in FTCD.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable