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Merck

F1056

Sigma-Aldrich

ヒト血漿フィブロネクチン

lyophilized powder, BioReagent, suitable for cell culture, ≥95% (SDS-PAGE)

別名:

フィブロネクチン 溶液 ヒト線維芽細胞由来

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About This Item

EC Number:
MDL番号:
UNSPSCコード:
12352202
NACRES:
NA.75

由来生物

human plasma

品質水準

無菌性

sterile

製品種目

BioReagent

アッセイ

≥95% (SDS-PAGE)

形状

lyophilized powder

包装

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

テクニック

cell culture | mammalian: suitable

表面被覆率

1‑5 μg/cm2

溶解性

H2O: 1 mg/mL at 37 °C

UniProtアクセッション番号

結合特異性

Peptide Source: Collagen

輸送温度

dry ice

保管温度

−20°C

遺伝子情報

human ... FN1(2335)

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関連するカテゴリー

詳細

Fibronectin is a class of high-molecular-weight adhesive glycoprotein, predominant in extracellular matrix (ECM) and is widely distributed in tissue and blood. The FN gene, which is highly conserved, gives rise to a minimum of 20 isoforms through alternative splicing in humans. These isoforms include notable variants such as cellular FN and plasma FN. Plasma FN is primarily synthesized in the liver, whereas cellular FN which represents only 5% of the circulating fibronectins is locally produced by various cell types.

アプリケーション

Fibronectin human plasma has been used:
  • in neural selection medium to coat tissue culture surface for neuronal differentiation
  • to coat culture vessels for characterization of iPSC and iPSC‑derived BMEC in 2D cell culture
  • to coat culture supports for endothelial cell culture

生物化学的/生理学的作用

Fibronectin plays a major role in biological processes. It interacts with surface receptors and other major ECM proteins like collagen to mediate cellular signals. Fibronectin is involved in a variety of cellular functions, including adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation. This glycoprotein can bind to integrins, collagens, fibrins and glycosaminoglycans (GAGs). FN plays a crucial role in promoting cell cycle progression and proliferation. This interaction influences cyclins and immediate early response genes, contributing to cellular processes related to cell division and growth. FN is also involved in cell survival and protection from apoptosis in various cell types, including epithelial and endothelial cells and leukocytes. FN serves as a critical factor in promoting cell survival and preventing programmed cell death in these cellular contexts. Mutations in the FN gene are associated with FN glomerulopathy and spondylometaphyseal dysplasia. Fibronectin plays a significant role in the wound healing process, and in extracellular matrix (ECM) assembly, hence is considered suitable for tissue engineering applications.

注意

再構成した溶液は作業用アリコートに分配して-20°C以下の温度で保存してください。再構成したフィブロネクチンのボルテックス攪拌や過剰な攪拌、凍結と誘拐の繰り返しはお薦めできません。

調製ノート

本製品は、TrisバッファでpH 7.5に保った塩溶液を凍結乾燥したものです。1 mg/mLで水に可溶であり、37°Cで最低でも30分間溶解させてください。少量の不溶解物が残るかもしれませんが、性能には影響しません。蛋白の同質性は免疫電気泳動法で確認されており、内毒素試験も行われています。使用した原材料に対してはHIV、B型肝炎、C型肝炎テストが行われて陰性であることが確認されています。

フィブロネクチンは、コーティング培地表面上で無菌平衡塩類溶液を用いて希釈後、最低下の量を用いてコーティングします。その後、表面を45分間、室温で乾燥させます。2~8°Cで2~4週間保存できます。

免責事項

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

保管分類コード

11 - Combustible Solids

WGK

WGK 3

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, type N95 (US)


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Kunal Sharma et al.
eLife, 10 (2021-07-06)
Uropathogenic Escherichia coli (UPEC) proliferate within superficial bladder umbrella cells to form intracellular bacterial communities (IBCs) during early stages of urinary tract infections. However, the dynamic responses of IBCs to host stresses and antibiotic therapy are difficult to assess in
Jianfei He et al.
Redox biology, 36, 101631-101631 (2020-08-19)
Anastellin (AN), a fragment of the first type III module in fibronectin (FN), initiates formation of superfibronectin, a polymer which resembles the native cell-derived fibrillar FN found in the extracellular matrix of many tissues, but which displays remarkably different functional
Sean M Anderson et al.
Biomaterials, 30(27), 4618-4628 (2009-06-23)
Growth factors are a class of signaling proteins that direct cell fate through interaction with cell-surface receptors. Although a myriad of possible cell fates stems from a growth factor binding to its receptor, the signaling cascades that result in one
Xiao-Qiang Yan et al.
Molecules (Basel, Switzerland), 24(9) (2019-05-06)
Cyclooxygenase-2 (COX-2) as a rate-limiting metabolism enzyme of arachidonic acid has been found to be implicated in tumor occurrence, angiogenesis, metastasis as well as apoptosis inhibition, regarded as an attractive therapeutic target for cancer therapy. In our research, a series
Bram Piersma et al.
Cell and tissue research, 374(1), 165-175 (2018-05-05)
Mechanosensing of fibroblasts plays a key role in the development of fibrosis. So far, no effective treatments are available to treat this devastating disorder. Spectrins regulate cell morphology and are potential mechanosensors in a variety of non-erythroid cells, but little

資料

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

プロトコル

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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