FC010

ヒト血漿フィブロネクチン精製タンパク質

This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro.

• UNSPSCコード: 12352202
• eCl@ss: 32160405
• NACRES: NA.75

特性

• 由来生物: human
• 品質水準: 100; 300
• 無菌性: sterile; sterile-filtered
• アッセイ: ~95% (SDS-PAGE)
• 形状: liquid
• 分子量: 220 kDa
• メーカー／製品名: Chemicon^®
• 濃度: 1 mg/mL
• テクニック: cell culture | mammalian: suitable
• 表面被覆率: 2—10 μg/cm²
• 入力: sample type mesenchymal stem cell(s); sample type neural stem cell(s); sample type: human embryonic stem cell(s); sample type epithelial cells; sample type hematopoietic stem cell(s); sample type induced pluripotent stem cell(s); sample type pancreatic stem cell(s)
• NCBIアクセッション番号: NM_002026.2; NM_054034.2; NM_212474.1; NM_212475.1; NM_212476.1; NM_212478.1; NM_212482.1
• UniProtアクセッション番号: P02751
• 結合特異性: Peptide Source: Collagen; ; Peptide Source: Laminin
• 輸送温度: wet ice
• 保管温度: 2-8°C
• 遺伝子情報: human ... FN1(2335)

詳細

詳細

Fibronectins (FNs) are high-molecular-mass adhesive glycoproteins of the extracellular matrix (ECM) and body fluids. It is a dimer composed of two subunits linked by two disulfide bonds. Each monomer of FN consists of three types of repeating units: 12 FN repeats of type I (approximately 40 amino acids each), two type II repeats (around 60 amino acids), and 15-17 type III repeats (approximately 90 amino acids). These repeating units contribute to the structural and functional diversity of fibronectin molecules.

アプリケーション

Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads.

生物化学的／生理学的作用

Fibronectin plays a crucial role in mediating diverse cellular interactions and is involved in various processes, including cell adhesion, the establishment and maintenance of cell morphology, cell migration, growth, and differentiation. It interacts with several extracellular matrix (ECM) and cell surface proteins, such as collagen, heparin, fibrin, and specific cell membrane receptors. Fibronectin can also serve as a ligand for multiple integrins, including the well-known fibronectin receptor alpha5-beta1, which binds to the arginylglycylaspartic acid (RGD) sequence in the repeat III-10 region of fibronectin. This extensive network of interactions highlights fibronectin′s significance in regulating cellular behavior and mediating tissue function.

物理的形状

50 mMトリス-緩衝生理食塩水（TBS）、pH 7.5、無菌ろ過液（保存剤無添加）に溶解。

保管および安定性

保存期間は2～8℃で受領日から最長6ヵ月間です。 冷凍しないでください。

アナリシスノート

還元条件下では220 kDaの二重バンドが存在します。hFNは、ゼラチンアガロースを用いたアフィニティークロマトグラフィーと、それに続くヘパリンアガロースを用いたクロマトグラフィーによって精製されます。ドナー由来血漿はスクリーニング済みであり、HIV、HTLV、B型およびC型肝炎に対して陰性であることが示されています。

その他情報

Suggested Procedure for Coating Cell Cultureware

1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm²) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm².

2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area.

3.Introduce the proper CO2 atmosphere, if required.

4.Add the calculated amount of HFN to each culture vessel.

5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes.

6.Remove residual balanced salt solution before proceeding with standard cell culture procedures

法的情報

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責事項

メルクのカタログまたは製品に添付されたメルクのその他の文書に記載されていない場合、メルクの製品は研究用途のみを目的としているため、他のいかなる目的にも使用することはできません。このような目的としては、未承認の商業用途、in vitroの診断用途、ex vivoあるいはin vivoの治療用途、またはヒトあるいは動物へのあらゆる種類の消費あるいは適用などがありますが、これらに限定されません。

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3