F0300

抗線維芽細胞増殖因子受容体2, 細胞質 ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗FGFR-2抗体
• MDL番号: MFCD02096344
• UNSPSCコード: 51111800
• NACRES: NA.41
• 結合体: unconjugated
• application: IHC (p); WB
• クローン: polyclonal
• 化学種の反応性: human
• citations: 10
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using trypsin-digested, human tissue sections; western blot: 0.25-0.5 μg/mL using extracts/lysates of FGFR-2 transfected cells

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 115-125 kDa (doublet)
• 化学種の反応性: human
• 強化検証: recombinant expression; Learn more about Antibody Enhanced Validation
• テクニック: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using trypsin-digested, human tissue sections; western blot: 0.25-0.5 μg/mL using extracts/lysates of FGFR-2 transfected cells
• UniProtアクセッション番号: P21802
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... FGFR2(2263)

詳細

詳細

Fibroblast growth factors receptors (FGFRs) are members of the tyrosine kinase family of growth factor receptors. They are glycosylated 110-150 k Da proteins that are constructed of an extracellular ligand binding region with either two or typically three immunoglobulin (Ig)-like domains and an eight amino acid ′acidic box′, a transmembrane region and a cytoplasmic split tyrosine kinase domain that is activated following ligand binding and receptor dimerization. At the mRNA level, FGFR-2 is highly expressed in developing human tissues including the brain (preferentially in glial cells), choroid plexus, skin, lung, kidney and bone. It is widely expressed in many adult human and animal tissues. It may be found in several anchorage-dependent cells, such as normal and malignant breast cancer cells.

免疫原

ヒトFGFR-2(bek)の細胞質領域のアミノ酸809-821に相当する合成ペプチド。

アプリケーション

Anti-Fibroblast Growth Factor Receptor-2 has been used in immunoblotting, immunoprecipitation, immunohistochemistry,western blotting.

Anti-Fibroblast Growth Factor Receptor-2, Cytoplasmic antibody produced in rabbit was used for immunohistochemistry of free-floating rat brain tissue sections.

生物化学的／生理学的作用

Fibroblast growth factors (FGFs) and their receptors, FGFRs, are important regulators of cell proliferation, migration, tissue repair and differentiation during vertebrate development. FGFR-2 isoforms are expressed exclusively on cells of epithelial origin and are involved in tumor progression in thyroid cancers. Abnormalities in FGFR-2 expressions have been observed in skin, bone and cancer pathologies.

Fibroblast growth factors receptors (FGFRs) mediated signal transduction requires dimerization or oligomerization and autophosphorylation of the receptors through their tyrosine kinase domain. Mutations in the extracellular domain of FGFR-2 causes Crouzon, as well as other craniosynostosis syndromes (e.g. Pfeiffer′s, Apert′s, Jackson-Weiss′), disorders of human skeletal development.

物理的形状

0.01M PBS溶液 (pH 7.4, 1%BSA, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)